Towards map-based cloning of FB_Mfu10 : identification of a receptor-like kinase candidate gene underlying the Malus fusca fire blight resistance locus on linkage group 10

Molecular Breeding : New Strategies in Plant Improvement
Ofere Francis EmeriewenAndreas Peil

Abstract

Breeding for resistance against the destructive fire blight disease of apples is the most sustainable strategy to control the menace of this disease, and has become increasingly important in European apple breeding programs. Since most cultivars are susceptible, wild accessions have been explored for resistance with quantitative trait loci detected in a few wild species. Fire blight resistance of Malus fusca was described following phenotypic evaluations with a C-type strain of Erwinia amylovora, Ea222_JKI, and the detection of a major QTL on chromosome 10 (Mfu10) of this crabapple. The stability of the resistance of M. fusca and Mfu10 has been evaluated using two other strains, the highly aggressive Canadian S-type strain-Ea3049, and the avrRpt2EA mutant-ZYRKD3-1, both of which overcome the resistance of Malus ×robusta 5, a wild species accession with an already described fire blight resistance gene. To pave the way for positional cloning of the underlying fire blight resistance gene of M. fusca, we have fine mapped the QTL region on linkage group 10 using 1888 individuals and 23 newly developed molecular markers, thus delimiting the interval of interest to 0.33 cM between markers FR39G5T7xT7y/FR24N24RP and FRMf7358424/FR46H22...Continue Reading

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Datasets Mentioned

BETA
MAL0045

Methods Mentioned

BETA
PCR
Illumina sequencing

Software Mentioned

FGENESH
QTL
BLAST
ExPASy PROSITE
MAP
KmerGenie
Trim Galore
Primer
JOINMAP
ExPASy

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