Towards Mapping Mouse Metabolic Tissue Atlas by Mid-Infrared Imaging with Heavy Water Labeling.

Advanced Science
Xinwen LiuWei Min

Abstract

Understanding metabolism is of great significance to decipher various physiological and pathogenic processes. While great progress has been made to profile gene expression, how to capture organ-, tissue-, and cell-type-specific metabolic profile (i.e., metabolic tissue atlas) in complex mammalian systems is lagging behind, largely owing to the lack of metabolic imaging tools with high resolution and high throughput. Here, the authors applied mid-infrared imaging coupled with heavy water (D2 O) metabolic labeling to a scope of mouse organs and tissues. The premise is that, as D2 O participates in the biosynthesis of various macromolecules, the resulting broad C-D vibrational spectrum should interrogate a wide range of metabolic pathways. Applying multivariate analysis to the C-D spectrum, the authors successfully identified both inter-organ and intra-tissue metabolic signatures of mice. A large-scale metabolic atlas map between different organs from the same mice is thus generated. Moreover, leveraging the power of unsupervised clustering methods, spatially-resolved metabolic signatures of brain tissues are discovered, revealing tissue and cell-type specific metabolic profile in situ. As a demonstration of this technique, the au...Continue Reading

References

Apr 1, 1991·Clinical Science·P J Jones, S T Leatherdale
Feb 1, 1995·The American Journal of Physiology·Y SunC B Smith
Aug 1, 1951·The American Journal of Medicine·R G GOULD
Aug 18, 2004·Progress in Neurobiology·Maria ErecinskaIan A Silver
Mar 29, 2006·Biochimica Et Biophysica Acta·Robert BuschMarc K Hellerstein
Sep 23, 2006·Physiology·Xuemin Wang, Christopher G Proud
Dec 15, 2007·Nature Protocols·Robert BuschMarc K Hellerstein
May 7, 2009·Cell Metabolism·Bei B ZhangCai Li
Oct 1, 2009·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Sadie L Hebert, K Sreekumaran Nair
Jan 17, 2012·Nature·Matthew L SteinhauserClaude P Lechene
Sep 27, 2012·Cancer Discovery·Jason R Cantor, David M Sabatini
Oct 16, 2012·Biochemical Pharmacology·Luca PersanoGiampietro Viola
May 28, 2013·Biochimica Et Biophysica Acta·Stephen F PrevisThomas P Roddy
Aug 24, 2013·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Geoff WarnockAlain R E G Plenevaux
May 2, 2014·Frontiers in Oncology·Camillo PortaAlessandra Mosca
Oct 27, 2015·Frontiers in Behavioral Neuroscience·Fiorenza StagniRenata Bartesaghi
Apr 8, 2016·Genome Biology·Rhonda Bacher, Christina Kendziorski
Nov 3, 2016·Nature Reviews. Clinical Oncology·Michelle M KimSriram Venneti
Jul 29, 2016·Nature Methods·Judith WeberSarah E Bohndiek
Sep 21, 2016·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Masaru Miyagi, Takhar Kasumov
Dec 14, 2016·Chemical Reviews·Alexandre Dazzi, Craig B Prater
Jan 25, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sevgi Türker-Kaya, Christian W Huck
May 29, 2018·Neuroscience Letters·Parthiv Haldipur, Kathleen J Millen
Aug 8, 2018·Nature Communications·Lingyan ShiWei Min
Dec 12, 2018·Nature Biotechnology·Etienne BechtEvan W Newell
Apr 27, 2019·Frontiers in Genetics·Geng ChenTieliu Shi
Aug 20, 2019·EMBO Reports·Judit Castillo-ArmengolIsabel C Lopez-Mejia
Dec 25, 2019·Nature Biotechnology·Miguel A PleitezVasilis Ntziachristos
Jan 3, 2020·Nature·Ioachim PupezaFerenc Krausz
Feb 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Martin SchnellRohit Bhargava
Jun 22, 2020·Annals of Nutrition & Metabolism·Pascal Steiner
Jul 1, 2020·Nature Methods·Lixue ShiWei Min
Oct 24, 2020·Trends in Genetics : TIG·Richard W Carthew

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