Towards profiling the gene expression of fibroblasts from atopic dermatitis patients: human 8K complementary DNA microarray

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
Y-D ParkJ-M Yang

Abstract

High-throughput technologies, including DNA-chip array, have been used to search for the genes that are dysregulated in human diseases. The atopic dermatitis (AD)-associated genes are gradually being reported; however, the differentially altered gene expression profiles of atopic fibroblasts have not been well elucidated. We wanted to gain more insights into AD and to find candidate genes, especially in regards to the role of fibroblasts in the pathogenesis of AD. cDNA microarray (8K) profiling of the primary cultured AD patients-derived fibroblasts was conducted by a pooling method of the recruited 22 normal controls, the 10 extrinsic type (ADe) patients and the 10 intrinsic type (ADi) patients. SAM analysis of the microarray results (2-fold cut-off) was conducted to select the candidate genes. Quantification by real-time PCRs confirmed the array data in the randomized paired samples (normal vs. ADe n=10; normal vs. ADi n=10). We listed the 22 up-regulated and 95 down-regulated genes in the AD fibroblasts. Real-time PCR results showed that several genes such as hyaluronan synthase 2 (HAS2), TNF-alpha-induced protein 6 (TNFAIP6) and IL-8 were matched with the array results with statistical significance. These results suggest ge...Continue Reading

References

May 1, 1995·International Archives of Allergy and Immunology·H U SimonK Blaser
Feb 1, 1994·Archives of Disease in Childhood·H Kimata, I Lindley
Jun 12, 1998·International Archives of Allergy and Immunology·U LippertB M Henz
Dec 5, 2000·Current Opinion in Immunology·C A AkdisK Blaser
Jan 4, 2001·The New England Journal of Medicine·A B Kay
Jan 11, 2001·The New England Journal of Medicine·A B Kay
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Feb 6, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Warren KnudsonCheryl B Knudson
Feb 6, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Paul W Noble
Apr 15, 2003·Journal of Cell Science·Caroline M Milner, Anthony J Day
Dec 6, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C-W JeongJ-M Yang
Dec 6, 2003·The Journal of Allergy and Clinical Immunology·Ichiro NomuraDonald Y m Leung
Jan 28, 2004·Nature Genetics·Xiaoqing LiuTiansen Li
Feb 22, 2005·The Journal of Biological Chemistry·Katri SaavalainenCarsten Carlberg
Jun 3, 2005·The New England Journal of Medicine·Hywel C Williams
Jun 21, 2005·Journal of the American Academy of Dermatology·Eric L Simpson, Jon M Hanifin
Jun 22, 2005·Journal of the American Academy of Dermatology·William Abramovits

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Citations

Oct 31, 2006·Omics : a Journal of Integrative Biology·Miroslav Blumenberg
Oct 5, 2010·Mediators of Inflammation·Daniela BraconiAnnalisa Santucci
May 16, 2009·Journal of Dermatological Science·Zhi-Rong LüFei Zou
Apr 28, 2009·Journal of Dermatological Science·Shona H WoodStuart D Carter
Oct 1, 2008·Journal of Dermatological Science·Zhi-Rong LüJun-Mo Yang
Dec 15, 2007·Toxicology and Applied Pharmacology·Noömi LombaertMicheline Kirsch-Volders
Mar 13, 2015·American Journal of Respiratory and Critical Care Medicine·Xiting YanGeoffrey L Chupp
Mar 1, 2008·Proteomics. Clinical Applications·Yong-Doo ParkJun-Mo Yang
Feb 23, 2019·Science Translational Medicine·Julia MatthiasChristina E Zielinski
Mar 20, 2020·Journal of Biomolecular Structure & Dynamics·Shang-Jun YinGuo-Ying Qian

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Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.