PMID: 6411468Aug 15, 1983Paper

Towards purification of the scrapie agent

European Journal of Biochemistry
H DiringerB Ehlers

Abstract

A method for the partial purification of scrapie infectivity from hamster brain is described. About a 100-1000-fold, 20-fold, and 200-fold enrichment in scrapie infectivity with respect to protein, RNA, and DNA content has been achieved using differential centrifugation, enzyme and detergent treatment. The inbred CLAC strain of hamsters used in our experiments contained about 10 times less infectivity in brain than has been found in randomly bred animals or other inbred strains.

References

Feb 1, 1977·The Journal of General Virology·R H Kimberlin, C Walker
Jun 1, 1976·The Journal of General Virology·M C Clarke, G C Millson
Jan 1, 1981·Advances in Experimental Medicine and Biology·K C KasperS B Prusiner
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S B PrusinerF R Masiarz
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S B PrusinerS P Cochran
Apr 1, 1982·Annals of Neurology·S B PrusinerH M Martinez
Jan 1, 1981·Acta Neuropathologica·P A MerzK Iqbal

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Citations

Jan 1, 1989·Acta Neuropathologica·Y KanekoK Yamaguchi
Jan 1, 1987·Acta Neuropathologica·S B PrusinerM P McKinley
Jan 1, 1984·Archives of Virology·H Diringer
Jul 7, 1984·Lancet·R G Rohwer
Aug 11, 1984·Lancet·H DiringerL Bode
Aug 30, 2008·Environmental Science & Technology·Glen T HinckleyJoel A Pedersen
May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·C Lemaire-VieilleJ Y Cesbron
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L ManuelidisE E Manuelidis
Aug 16, 2006·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·M EidenM H Groschup
Sep 10, 2014·Proteomics. Clinical Applications·Sarah PlumCaroline May
Apr 1, 1985·Cell·B OeschL E Hood
Jan 1, 1985·Microbiology and Immunology·M ShinagawaG Sato
Jun 1, 1983·Bioscience Reports·H Diringer, R H Kimberlin
Jan 17, 2016·Journal of Cellular Biochemistry·Sotirios Botsios, Laura Manuelidis
Dec 1, 1983·Nature·H DiringerR H Kimberlin
Jan 26, 2005·The Journal of Biological Chemistry·Lior HoronchikAlbert Taraboulos
Dec 1, 1986·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S B Prusiner
Mar 22, 2015·Expert Review of Proteomics·Roger A MooreSuzette A Priola
Aug 11, 2004·The Journal of General Virology·Anne BuschmannMartin H Groschup
May 17, 2000·The Journal of General Virology·T SweeneyM G Parada
Nov 1, 1987·Archives of Biochemistry and Biophysics·D C BoltonA Potempska
Feb 4, 2011·Analytical Chemistry·Christopher J SilvaJohn Mark Carter

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