Towards RIP using free-electron laser SFX data

Journal of Synchrotron Radiation
Lorenzo GalliMax H Nanao

Abstract

Here, it is shown that simulated native serial femtosecond crystallography (SFX) cathepsin B data can be phased by rapid ionization of sulfur atoms. Utilizing standard software adopted for radiation-damage-induced phasing (RIP), the effects on both substructure determination and phasing of the number of collected patterns and fluences are explored for experimental conditions already available at current free-electron laser facilities.

References

Nov 21, 2001·Protein Science : a Publication of the Protein Society·P PerssonJ Hajdu
Feb 11, 2003·Structure·Raimond B G RavelliSean McSweeney
Jul 24, 2003·Acta Crystallographica. Section D, Biological Crystallography·Gwyndaf EvansGérard Bricogne
Jul 13, 2004·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Stefan P Hau-RiegeAbraham Szoke
Apr 21, 2005·Journal of Synchrotron Radiation·Raimond B G RavelliSean McSweeney
Sep 1, 2005·Acta Crystallographica. Section D, Biological Crystallography·Max H NanaoRaimond B G Ravelli
Mar 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robin Leslie OwenElspeth F Garman
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·George M Sheldrick
Apr 15, 2010·Optics Express·Richard A KirianJames Holton
Feb 5, 2011·Nature·Henry N ChapmanJohn C H Spence
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Dec 21, 2011·Physical Review Letters·Sang-Kil SonRobin Santra
Jan 31, 2012·Nature Methods·Linda C JohanssonRichard Neutze
Apr 6, 2012·Journal of Applied Crystallography·Andrea Thorn, George M Sheldrick
Sep 6, 2012·Acta Crystallographica. Section D, Biological Crystallography·Daniele de Sanctis, Max H Nanao
Jun 26, 2013·Acta Crystallographica. Section D, Biological Crystallography·Thomas A WhiteHenry N Chapman
Nov 26, 2013·Nature·Thomas R M BarendsIlme Schlichting

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Citations

Mar 10, 2016·Acta Crystallographica. Section D, Structural Biology·Daniele de SanctisMax H Nanao
Nov 26, 2015·IUCrJ·Lorenzo GalliHenry N Chapman
Jan 23, 2016·Structural Dynamics·Bahige G AbdallahAlexandra Ros
Feb 28, 2015·Journal of Synchrotron Radiation·Elspeth F Garman, Martin Weik
May 5, 2016·Archives of Biochemistry and Biophysics·Jose M Martin-GarciaPetra Fromme
Nov 3, 2016·The Journal of Chemical Physics·Arie Landau, Nimrod Moiseyev
Apr 14, 2018·Acta Crystallographica. Section D, Structural Biology·Nicolas FoosMax H Nanao
May 24, 2019·The Journal of Physical Chemistry. a·Arie Landau, Idan Haritan

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