Towards the definition of a chimpanzee and human conserved CD6 domain 1 epitope recognized by T1 monoclonal antibody

Hybridoma
Ruby AlonsoEnrique Montero

Abstract

Scavenger receptor cysteine-rich (SRCR) domains are evolutionally conserved modules that display complex structures stabilized by key amino acids, while some other residues have evolved with a relative independence, thus allowing the functional diversity of these receptors. CD6, a highly glycosylated membrane protein predominantly expressed on lymphocytes, contains three SRCR domains. The lack of CD6 domain crystal structure has limited the characterization of the binding sites for the interacting molecules. The interaction between CD6 and its ligand, activated leukocyte-cell adhesion molecule (ALCAM)/CD166, through the membrane-proximal SRCR3 domain, has low affinity and involves conserved sites in both molecules mediating a cross-species binding. The CD6-ALCAM interaction has been involved in cell adhesion, maturation, regulation of activation, and survival processes, suggesting the potential relevance of this target for therapeutic interventions. Several anti-CD6 monoclonal antibodies (MAb) have been described but their affinity and epitope definition remain unclear. We found the murine and humanized T1 MAb versions have similar CD6 recognition profiles and affinity constants of about 6 x 10(8). These antibodies do not block...Continue Reading

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Citations

Apr 10, 2013·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Mafalda Pinto, Alexandre M Carmo
Jan 1, 2010·Arthritis·Ruby Alonso-RamirezYves Renaudineau
Sep 3, 2010·Journal of Autoimmunity·Ruby AlonsoYves Renaudineau
May 18, 2018·Immunology·Lee I GarnerMarion H Brown
Jan 20, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jian HuangPing Dai
Feb 18, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yan LiFeng Lin
Jan 9, 2019·Frontiers in Immunology·Carine M GonçalvesAlexandre M Carmo
Oct 19, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marion H Brown, Erica Lacey
Aug 19, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·David M KoflerDavid A Hafler
May 30, 2020·Expert Opinion on Biological Therapy·Sunil DograMurlidhar Rajagopalan

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