Towards the development and characterization of an easy handling sheet-like biohybrid bone substitute

Tissue Engineering. Part a
Timothée BaudequinCécile Legallais

Abstract

We designed a sheet-like bone substitute capable of adapting to different geometries and becoming a standard tissue-engineered process for bone surgery. Preosteoblastic cells were seeded on to a monolayer of calcium phosphate granules and cultured in a flat parallelepipedic cell culture chamber for 1 month. From the various diameters of the granules examined, the 80-200 μm group exhibited the most homogeneous performances regarding both biological (cell morphology, viability, differentiation, and simple metabolic activity) and mechanical (cohesion and stress-strain behavior) properties. This sheet was easy to handle after extraction from the culture chamber and showed versatile geometry and flexibility, making it easy to use for surgeons, especially for small defects of the maxillofacial area.

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Citations

Dec 2, 2017·Advanced Healthcare Materials·Timothée Baudequin, Maryam Tabrizian
Sep 10, 2018·ACS Biomaterials Science & Engineering·Alejandro Garcia GarciaCecile Legallais
Oct 14, 2021·The International Journal of Artificial Organs·Timothée BaudequinCécile Legallais

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Methods Mentioned

BETA
scanning electronic microscopy
fluorescence microscopy
Assay
Enzyme-linked Immunosorbent Assay
ELISA

Software Mentioned

MS Excel
CellProfiler

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