Towards understanding the biosynthetic pathway for ustilaginoidin mycotoxins in Ustilaginoidea virens

Environmental Microbiology
Yuejiao LiWenxian Sun

Abstract

Ustilaginoidins, toxic to plants, animals and human, are one of major types of mycotoxins produced by Ustilaginoidea virens. In this study, a gene cluster containing the polyketide synthase gene UvPKS1 was analysed via gene replacement and biochemical studies to determine ustilaginoidin biosynthetic pathway in U. virens. UvPKS1 was first proven to be responsible for the first step of ustilaginoidin biosynthesis, since neither ustilaginoidin derivatives nor intermediates were produced when UvPKS1 was deleted. Replacement of ugsO greatly reduced ustilaginoidin production but increased the ratios of dehydrogenated/hydrogenated ustilagioidin derivatives. The enhanced growth rate of the ΔugsO mutant indicates that accumulation of certain ustilaginoidin derivatives may adversely affect mycelial growth in U. virens. Deletion of ugsT encoding a putative MFS transporter disrupted the ability to generate ustilaginoidins. The ustilaginoidin derivatives produced in the ΔugsJ mutant all lack C3-methyl, indicating that UgsJ is responsible for C3-methylation. Only monomeric intermediates, such as 3-methyl-dihydro-nor-rubrofusarin, but no ustilaginoidin derivatives were generated in the ΔugsL mutant, indicating that UgsL is responsible for the...Continue Reading

References

Sep 1, 1988·Journal of Pharmacobio-dynamics·K KoyamaT Tsuruo
Apr 29, 1994·Biochemical Pharmacology·R F LudueńaS Iwasaki
Jul 1, 1996·Applied and Environmental Microbiology·A E DesjardinsR H Proctor
Jun 15, 1997·Nucleic Acids Research·M S SachsB S Margolin
Jun 19, 1998·The Journal of Antibiotics·N MorisakiS Iwasaki
Jun 29, 2000·Annual Review of Biochemistry·C KhoslaD E Cane
Feb 12, 2002·Critical Reviews in Food Science and Nutrition·Nelson DuránElisa Esposito
Nov 1, 2002·Fungal Genetics and Biology : FG & B·Pia LinnemannstönsBettina Tudzynski
Mar 7, 2003·Fungal Genetics and Biology : FG & B·Robert H ProctorAnne E Desjardins
Mar 26, 2003·Applied Microbiology and Biotechnology·D BhatnagarT E Cleveland
Sep 1, 1963·Chemical & Pharmaceutical Bulletin·S SHIBATAA OHTA
Dec 1, 1963·Chemical & Pharmaceutical Bulletin·S SHIBATA, Y OGIHARA
Mar 24, 2004·Micron : the International Research and Review Journal for Microscopy·Harald Claus
May 17, 2005·Fungal Genetics and Biology : FG & B·Chang Hyun KhangSeogchan Kang
Feb 8, 2006·Applied and Environmental Microbiology·Jung-Eun KimYin-Won Lee
Mar 7, 2006·Applied and Environmental Microbiology·Iffa Gaffoor, Frances Trail
Mar 16, 2006·Journal of Agricultural and Food Chemistry·Robert H ProctorRobert A E Butchko
Nov 23, 2006·Journal of Molecular Microbiology and Biotechnology·Milton H Saier, Qinghu Ren
Aug 21, 2007·International Journal of Food Microbiology·A E Desjardins, R H Proctor
Apr 14, 2009·Methods in Enzymology·Kira J Weissman
Apr 30, 2009·Molecular Microbiology·Philipp WiemannBettina Tudzynski
Jun 11, 2009·Angewandte Chemie·Christian Hertweck
Jun 18, 2010·Fungal Genetics and Biology : FG & B·Nora KhaldiNatalie D Fedorova
Feb 8, 2011·The Journal of Biological Chemistry·Rasmus J N FrandsenHenriette Giese
Dec 17, 2011·Applied Microbiology and Biotechnology·Helene C Eisenman, Arturo Casadevall
Jul 28, 2012·Molecular Plant-microbe Interactions : MPMI·Jon MenkeH Corby Kistler
Mar 4, 2014·Nature Biotechnology·Jeffry D Sander, J Keith Joung
Jun 3, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shiqiong LuLigang Zhou
Mar 18, 2015·Journal of Agricultural and Food Chemistry·Shiqiong LuLigang Zhou
May 8, 2015·Nucleic Acids Research·Tilmann WeberMarnix H Medema
Aug 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Peng FengChengshu Wang
Oct 17, 2015·Toxins·Jiajia MengGuozhen Zhang

❮ Previous
Next ❯

Citations

Dec 4, 2019·Angewandte Chemie·Vincent C FäsekeChristof Sparr
Nov 17, 2020·Natural Product Reports·Wolfgang Hüttel, Michael Müller

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.