PMID: 9003536Nov 11, 1996Paper

Towards validation of the Big Blue transgenic mouse mutagenesis assay: the mutational spectrum of ex vivo pinpoint mutant plaques

Mutation Research
H NishinoS S Sommer

Abstract

To explore further the origin of spontaneous mutations recovered with the Big Blue transgenic mouse mutagenesis assay, the spectrum of ex vivo mutations from pinpoint mutant plaques was determined and compared with the spectrum of putatively mouse-derived mutations from circular, mutant plaques. The entire lacI gene and lacZ operator region from 62 pinpoint blue plaques was sequenced. The observed mutational spectrum of pinpoint mutants differed significantly from that seen in circular mutants (p < 0.0001). Only four percent of the mutations were transitions at CpG sites whereas this type of mutation was the most common (35%) in circular mutants. Microdeletions/microinsertions were seen more frequently in pinpoint mutants relative to circular mutants. Four base pair deletion/insertion events at the E. coli hotspot tandem repeats were seen in 10 of 62 (16%) pinpoint mutants and minor hotspots of mutation were observed at bp 141 and 1110. The mutational spectrum of pinpoint mutants provides further evidence that most circular mutants originate in mouse.

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Citations

Jan 1, 1996·Environmental and Molecular Mutagenesis·H NishinoS S Sommer
Apr 16, 2004·DNA Repair·Christi A WalterRonald B Walter
May 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jicheng WangSteve S Sommer
Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jason H Bielas, John A Heddle
Sep 2, 2004·Molecular and Cellular Biology·Jessica HuamaniChristi A Walter
Dec 25, 2008·Environmental and Molecular Mutagenesis·Rory A CrabbeKathleen A Hill
Feb 2, 2010·Environmental and Molecular Mutagenesis·Tonya L WardKathleen A Hill
Aug 6, 2005·Mutation Research·Iain B LambertGeorge R Douglas
Oct 2, 2002·Environmental and Molecular Mutagenesis·Fumio YatagaiNobuhisa Fukunishi
Apr 7, 2000·Environmental Health Perspectives·B RihnG Keith

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