PMID: 8595871Jan 15, 1996Paper

Toxic and non-toxic strains of the cyanobacterium Microcystis aeruginosa contain sequences homologous to peptide synthetase genes

FEMS Microbiology Letters
K MeissnerThomas Börner

Abstract

Toxic strains of Microcystis aeruginosa produce cyclic heptatoxins (microcystins) that are believed to be synthesized non-ribosomally by peptide synthetases. We analysed toxin-producing and non-toxic strains of M. aeruginosa with respect to the presence of DNA sequences potentially encoding peptide synthetases. Hybridizations of genomic DNA of various M. aeruginosa strains with PCR-amplificated fragments possessing homologies to adenylate-forming domains of peptide synthetase genes provided first evidence for the existence of corresponding genes in cyanobacteria. Furthermore we isolated and sequenced from genomic libraries overlapping fragments of M. aeruginosa DNA with a total length of 2982 bp showing significant homology to genes encoding peptide synthetases and hybridizing exclusively with DNA from toxic strains. Our results indicate that both toxic and non-toxic strains of M. aeruginosa possess genes coding for peptide synthetases and that hepatotoxin-producing and non-toxic strains differ in their content of genes for specific peptide synthetases.

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Citations

Apr 5, 2005·Toxicon : Official Journal of the International Society on Toxinology·Janet M BensonLora E Fleming
Aug 15, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T TsutsumiY Ueno
Jul 17, 1998·Current Opinion in Chemical Biology·H D Mootz, M A Marahiel
Oct 24, 2002·Journal of Applied Microbiology·I V FouldsP A Horgen
Mar 15, 2005·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Ronald W ZurawellEllie E Prepas
May 27, 2008·Molecular Biology and Evolution·Guntram ChristiansenRainer Kurmayer
Jan 20, 2010·PloS One·Alan E WilsonOrlando Sarnelle
Nov 27, 2002·Applied and Environmental Microbiology·Judith A BakerDavid B McKay
Mar 4, 2000·Environmental Health Perspectives·B C HitzfeldD R Dietrich
Jan 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Anne RantalaKaarina Sivonen
Mar 2, 2005·Toxicology and Applied Pharmacology·Elke Dittmann, Thomas Börner
Mar 11, 2016·Environmental Science and Pollution Research International·Koji TomitaKen-Ichi Harada
Jan 15, 2014·Applied and Environmental Microbiology·Lin ZhuNanqin Gan
Jun 16, 2006·FEMS Microbiology Reviews·Martin Welker, Hans von Döhren
Jun 29, 1999·Journal of Bacteriology·B A NeilanT Börner

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