Toxic effects of dexamethasone on mouse testicular germ cells

Andrologia
M OrazizadehM Hashemitabar

Abstract

Exposure to glucocorticoids (GCs) leads to numerous changes in various biological systems including the reproductive system. This work evaluated effects of dexamethasone (Dex), a widely used GC, on mouse testicular germ cells. Experimental groups (E1-E3) received one of the following treatments daily for 7 days: 4, 7 and 10 mg kg(-1) Dex respectively. Control groups were treated with equivalent volumes of saline. Testicular histopathology, morphometric analysis and deoxy-UTP-digoxigenin nick end labeling (TUNEL) assessment were performed for evaluation of the toxic effects of Dex and detection of the apoptotic cells. The results showed that Dex induces histopathological alterations such as epithelial vacuolisation, atrophy and reduction in testicular spermatozoid. Morphometrical data showed that Dex significantly reduced tubular diameter and epithelial height (P < 0.05). Johnsen's scoring also showed poor spermatogenesis in E2 and E3 groups (P < 0.05). Apoptotic index of germ cells was significantly increased in E2 (18.9% versus 1.76%, P < 0.01) and E3 (24.6 versus 1.76%, P < 0.001) groups. It is concluded that Dex acts as testicular toxicant and that further studies are needed to establish its mechanism of action upon spermato...Continue Reading

References

Sep 1, 1984·Canadian Journal of Physiology and Pharmacology·M BernierJ R Ducharme
Dec 1, 1980·The American Journal of Anatomy·H Elias, D M Hyde
Jul 1, 1993·Toxicology and Applied Pharmacology·K W HewM J Welsh
Jan 1, 1993·Toxicologic Pathology·K P LeeR Valentine
Mar 2, 1999·Reviews of Reproduction·A P Sinha Hikim, R S Swerdloff
Jun 23, 1999·Reproductive Toxicology·H Y SonJ K Roh
Nov 24, 1999·Reproductive Toxicology·J C KimM K Chung
Jul 25, 2000·Toxicology and Applied Pharmacology·O J D'Cruz, F M Uckun
Oct 24, 2000·Proceedings of the Society for Experimental Biology and Medicine·K BoekelheideH A Schoenfeld
Dec 26, 2001·Endocrinology·Hui-Bao GaoMatthew P Hardy
Feb 13, 2003·Molecular and Cellular Endocrinology·Hui-Bao GaoMatthew P Hardy
Mar 14, 2003·Journal of Andrology·Makoto KimuraTaiji Tsukamoto
Aug 5, 2005·Cell and Tissue Research·Matthew P HardyChantal Sottas
Nov 23, 2005·The Journal of Cell Biology·Nisrine El ChamiDaniel C Régnier
Jan 13, 2006·British Journal of Pharmacology·Julia C Buckingham

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Citations

Mar 8, 2012·Journal of Assisted Reproduction and Genetics·Mehri MirhoseiniLayasadat Khorsandi
Aug 21, 2013·Journal of Assisted Reproduction and Genetics·Ali Reza TalebiMahnaz Moridian
Apr 11, 2014·Iranian Red Crescent Medical Journal·Mehri MirhoseiniJavad Mohammadi Asl
Feb 12, 2014·Journal of Assisted Reproduction and Genetics·Mahmoud OrazizadehErfan Daneshi
Jul 13, 2014·Chemico-biological Interactions·Mahmoud OrazizadehLayasadat Khorsandi
Nov 3, 2012·Pharmaceutical Biology·Layasadat KhorsandiSoheila Khaghani
Jan 7, 2015·Reproduction, Fertility, and Development·Monika Hułas-StasiakEwa Tomaszewska
Jan 1, 2013·Cells·Amanda L Gruver-Yates, John A Cidlowski
Sep 2, 2016·PloS One·Fátima RamalhosaAna Franky Carvalho
Jun 17, 2020·Andrologia·Ayodeji Folorunsho Ajayi, Roland Eghoghosoa Akhigbe
Jul 18, 2012·The Journal of Clinical Endocrinology and Metabolism·Marine PoulainGabriel Livera
Nov 10, 2020·Drug and Chemical Toxicology·Malek Soleimani MehranjaniFirouzeh Sadeghzadeh
Apr 30, 2021·Saudi Journal of Biological Sciences·Sara Mohamed SaberAmel Ramadan Omar
May 26, 2021·Molecular Reproduction and Development·Li RenBin He

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