Toxic effects of lipid-mediated gene transfer in ventral mesencephalic explant cultures

Basic & Clinical Pharmacology & Toxicology
Matthias BauerMarius Ueffing

Abstract

Adverse effects of cDNA and oligonucleotide delivery methods have not yet been systematically analyzed. We introduce a protocol to monitor toxic effects of two non-viral lipid-based gene delivery protocols using CNS primary tissue. Cell membrane damage was monitored by quantifying cellular uptake of propidium iodide and release of cytosolic lactate dehydrogenase to the culture medium. Using a liposomal transfection reagent, cell membrane damage was already seen 24 hr after transfection. Nestin-positive target cells, which were used as morphological correlate, were severely diminished in some areas of the cultures after liposomal transfection. In contrast, the non-liposomal transfection reagent revealed no signs of toxicity. This approach provides easily accessible information of transfection-associated toxicity and appears suitable for prescreening of transfection reagents.

References

Jan 26, 1989·Nature·P L Felgner, G M Ringold
Dec 1, 1981·Journal of Neuroscience Methods·B H Gähwiler
Apr 14, 1997·Neuroreport·A McQuillinH M Gurling
Feb 12, 1999·Brain Research. Brain Research Protocols·J NorabergJ Zimmer
Nov 13, 1999·Nature Cell Biology·I GaidarovJ H Keen
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·S Chesnoy, L Huang
Aug 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·Anna M Krichevsky, Kenneth S Kosik
Feb 26, 2004·Lancet Neurology·Beverly L Davidson, Henry L Paulson
Mar 23, 2004·Current Pharmaceutical Design·Yoko Shoji, Hideki Nakashima
Feb 1, 1946·The Anatomical Record·M ABERCROMBIE

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Citations

Mar 30, 2010·Journal of Neural Transmission·David B O'SullivanAideen M Sullivan
Apr 30, 2011·Briefings in Functional Genomics·Ming JiangMichael Howell
Jul 25, 2009·American Journal of Physiology. Cell Physiology·Changgong WuDorothy E Vatner
Dec 4, 2012·International Journal of Molecular Sciences·Marisa L Martin-Fernandez, David T Clarke
Apr 3, 2009·Molecular and Cellular Neurosciences·Ellen L SuggateAnn Logan
Aug 10, 2011·Journal of Cellular Biochemistry·Franziska MussbachSiegmund Reissmann
Jun 30, 2007·Current Biology : CB·Jacob M SchrøderLotte B Pedersen
Sep 17, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·So Jin LeeKwangmeyung Kim
Nov 22, 2016·Tissue Engineering. Part C, Methods·Li Min TayChenjie Xu
Jul 3, 2009·Journal of Neurosurgery. Pediatrics·Gad Bar-JosephJoseph N Guilburd
Nov 5, 2019·Frontiers in Neuroscience·Albrecht StrohJohannes Boltze
Jan 22, 2020·Journal of Nanobiotechnology·Ami JoRichey M Davis
Nov 30, 2019·BioMed Research International·María V Villagrana-EscareñoJaime Ruíz-García
Jan 14, 2021·Pest Management Science·Kristopher SilverKun Yan Zhu

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