Toxicity and pathology of crude broth filtrate produced by Hirsutella thompsonii var. thompsonii in shake culture

Journal of Invertebrate Pathology
Alain VeyC W McCoy

Abstract

Metabolic products secreted by the fungal mycelia of Hirsutella thompsonii var. thompsonii (CBS 556.77D) in a defined culture broth in shake culture were tested for toxicity to Galleria mellonella larvae and Drosophila melanogaster adults via injection and per os application, respectively. In addition, the toxic effect of broth filtrate was observed in vitro in a cell line of Bombyx mori. Czapek-Dox broth fortified with 1% yeast extract stimulated more rapid mycelial growth and correspondingly more toxin production in time. At 25-30 degrees C, metabolic toxin(s) was detected in broth via bioassay at about 4-5 days postinoculation when mycelial biomass reached 5 mg/ml (dry wt). At these temperatures, biological activity of the filtrate peaked at about 8-10 days when mycelial growth reached a maximum (10 mg/ml, dry wt). This suggests a positive relationship between toxic metabolite and mycelial production. After 10 days, the toxicity of the filtrate appeared to decline gradually. Pathogenicity symptoms of the metabolites developed slowly in both G. mellonella and D. melanogaster. Early signs of lethargy appeared at 4 days postinjection and cumulative mortality of G. mellonella larvae was low after 1 week; however, the percentage ...Continue Reading

Citations

Nov 17, 2012·Insect Biochemistry and Molecular Biology·Miriam OlombradaLucía García-Ortega
Aug 3, 2010·Journal of Invertebrate Pathology·A Ortiz-UrquizaE Quesada-Moraga
Sep 29, 2004·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Rosa FuguetAlain Vey
Feb 8, 2005·Applied Microbiology and Biotechnology·Ali AsaffMayra de la Torre
Sep 28, 2006·Experimental & Applied Acarology·Luciana Savoi Rossi-Zalaf, Sérgio Batista Alves
Jan 1, 2013·Insects·Elías Herrero-GalánMercedes Oñaderra
Feb 24, 2017·Toxins·Miriam OlombradaLucía García-Ortega
Jun 1, 1995·Microbiology·Isabelle Mazet, Alain Vey
Mar 19, 2014·Toxicon : Official Journal of the International Society on Toxinology·Miriam OlombradaLucía García-Ortega
Oct 24, 1998·Journal of Invertebrate Pathology·D G BouciasJ C Pendland

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