Toxicity assessment of Gryllus bimaculatus (a type of cricket) glycosaminoglycan.

Toxicological Research
Mi Young AhnYou Young Jo

Abstract

We performed general toxicity studies of Gryllus bimaculatus (two-spotted cricket) glycosaminoglycan (GbG), including a single, 4-week repeated oral dose toxicity test in ICR mice, and short-term genotoxicity tests. The mutagenic potential of the purified GbG was non-genotoxic when it was evaluated using short-term genotoxicity tests, namely Ames, chromosome aberration (CA), and micronuclei (MN) tests. In Salmonella typhimurium and Escherichia coli assays, GbG did not produce any mutagenic response in the absence or presence of S9 mix with five bacterial strains (TA98, TA100, TA1535, TA1537, and WP2uvrA). Chromosome aberration test showed that GbG had no significant effect on Chinese hamster ovary (CHO) cells. In mouse micronuclei tests after twice oral treatments per day for two days, no significant alteration in the occurrence of micronucleated polychromatic erythrocytes was observed in ICR male mice intraperitoneally administered with GbG at doses of 15.63, 31.25, or 62.50 mg/kg. These results indicate that GbG has no mutagenic potential in these in vitro and in vivo systems. After GbG was orally administered at doses of 20, 40, 80, and 160 mg/kg for a single oral dose toxicity study and at 0, 40, 80, and 160 mg/kg bw/day fo...Continue Reading

References

Oct 30, 2004·Journal of Toxicology and Environmental Health. Part a·Mi Young AhnByung Mu Lee
Dec 6, 2005·Journal of Toxicology and Environmental Health. Part a·Mi Young AhnByung Mu Lee
Dec 8, 2011·Regulatory Toxicology and Pharmacology : RTP·Lourdes CanutLaura Vicente
Apr 25, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Niccolò MiragliaMadhu G Soni
Mar 23, 2017·International Journal of Molecular Sciences·Mi Young AhnKun-Koo Park
Jan 7, 2019·BMC Cancer·Mi Young AhnKun-Koo Park

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Citations

Dec 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Keya GangulyKi-Taek Lim

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Methods Mentioned

BETA
chromosomal aberrations

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