Toxicity, biodistribution, and ex vivo MRI detection of intravenously injected cationized ferritin

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
Scott C BeemanK M Bennett

Abstract

The goal of the work was to establish the toxicity and biodistribution of the superparamagnetic protein cationized ferritin (CF) after intravenous injection. Intravenously injected CF has been used to target the extracellular matrix with high specificity in the kidney glomerulus, allowing measurements of individual glomeruli using T2*-weighted MRI. For the routine use of CF as an extracellular matrix-specific tracer, it is important to determine whether CF is toxic. In this work, we investigated the renal and hepatic toxicity, leukocyte count, and clearance of intravenously injected CF. Furthermore, we studied CF labeling in several organs using MRI and immunohistochemistry. Serum measurements of biomarkers suggest that intravenous injection of CF is neither nephrotoxic nor hepatotoxic and does not increase leukocyte counts in healthy rats at a dose of 5.75 mg/100 g. In addition to known glomerular labeling, confocal and MRI suggest that intravenously injected CF labels the extracellular matrix of the hepatic sinusoid, extracellular glycocalyx of alveolar endothelial cells, and macrophages in the spleen. Liver T2* values suggest that CF is cleared by 7 days after injection. These results suggest that CF may serve as a useful co...Continue Reading

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Citations

Sep 12, 2013·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·John F BertramKevin M Bennett
Nov 12, 2013·Advances in Chronic Kidney Disease·Jennifer R CharltonKevin M Bennett
Apr 26, 2014·Contrast Media & Molecular Imaging·M Veronica Clavijo JordanKevin M Bennett
Feb 8, 2013·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Scott C BeemanKevin M Bennett
Sep 30, 2014·IEEE Transactions on Bio-medical Engineering·Min ZhangKevin Bennett
Apr 14, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Jennifer R CharltonKevin M Bennett
Mar 29, 2014·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Joseph F GeorgesPeter Nakaji
Jul 25, 2019·American Journal of Physiology. Renal Physiology·Edwin J BaldelomarKevin M Bennett
Nov 3, 2017·American Journal of Physiology. Renal Physiology·Edwin J BaldelomarKevin M Bennett
May 24, 2020·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Neda ParvinKevin M Bennett
Mar 22, 2014·American Journal of Physiology. Renal Physiology·Scott C BeemanKevin M Bennett
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Feb 17, 2015·Chemical Reviews·Günther JutzAlexander Böker
Jul 21, 2021·American Journal of Physiology. Renal Physiology·Jennifer R CharltonKevin M Bennett

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