Toxicity of Bacillus thuringiensis Spore and Crystal Protein to Resistant Diamondback Moth (Plutella xylostella).

Applied and Environmental Microbiology
J D TangM Peferoen

Abstract

A colony of Plutella xylostella from crucifer fields in Florida was used in mortality bioassays with HD-1 spore, CryIA(a), CryIA(b), CryIA(c), CryIB, CryIC, CryID, CryIE, or CryIIA. The data revealed high levels of field-evolved resistance to HD-1 spore and all CryIA protoxins and no resistance to CryIB, CryIC, or CryID. CryIE and CryIIA were essentially not toxic. When HD-1 spore was combined 1:1 with protoxin and fed to susceptible larvae, spore synergized the activity of CryIA and CryIC 5- to 8-fold and 1.7-fold, respectively, and did not synergize the mortality of CryIIA. When fed to Florida larvae, spore failed to synergize the activity of all three CryIA protoxins, synergized the activity of CryIC 5.3-fold, and did not synergize the mortality for CryIIA. Binding studies with CryIA(b), CryIB, and CryIC were performed to determine possible mechanisms of resistance. The two techniques used were (i) binding of biotinylated toxin to tissue sections of larval midguts and (ii) binding of biotinylated toxin to brush border membrane vesicles prepared from whole larvae. Both showed dramatically reduced binding of CryIA(b) in resistant larvae compared with that in susceptible larvae but no differences in binding of CryIB or CryIC.

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Citations

Sep 20, 1997·The Journal of Biological Chemistry·B OppertW H McGaughey
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·B E TabashnikJ Ferré
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·B E TabashnikD G Heckel
Oct 13, 2006·Current Microbiology·Sheila NathanNor M Mahadi
Jun 12, 2013·Nature Biotechnology·Bruce E TabashnikYves Carrière
Sep 26, 2001·Applied and Environmental Microbiology·J González-CabreraJ Ferré
Dec 1, 2001·Annual Review of Entomology·Juan Ferré, Jeroen Van Rie
Mar 4, 2000·Nature Biotechnology·A M SheltonE D Earle
Jun 27, 2001·Applied and Environmental Microbiology·Y B LiuN Crickmore
Mar 24, 2017·Journal of Economic Entomology·B PetersonJ Van den Berg
Nov 5, 1997·Nature Biotechnology·R T Roush, A M Shelton
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·M C WirthB A Federici
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Jun 1, 1997·Applied and Environmental Microbiology·Y Liu, B E Tabashnik
Feb 20, 2010·Journal of Invertebrate Pathology·Youjing GongYidong Wu

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