PMID: 8995467Nov 1, 1996Paper

Toxicity of ethanol in low concentrations. Experimental evaluation in cell culture

Acta Radiologica
E TapaniE Lehtonen

Abstract

To define the threshold ethanol concentration that is toxic to cultured cells. Three malignant cell lines and freshly isolated normal rat hepatocytes were exposed to 0-50% (vol.) ethanol (concentrations used were 0, 5, 10, 15, 20, 25, 30, 40 and 50%) on tissue culture plates for 0.25-60 min (exposure times used were 0.25, 5, 10, 20, 30, 40 50 and 60 min). Cytotoxicity was estimated by trypan blue exclusion test and from 3H-thymidine incorporation. All cells were killed by a 15-s exposure to 30-40% ethanol while a concentration as low as 15-20% gave a total response after 5-10-min exposures. After a one-hour exposure of F9 carcinoma cells and hepatocytes, a total or nearly total response was achieved with 10% ethanol. The cytotoxic effect was thus dependent both on the exposure time and on the concentration of ethanol. There were no significant differences in ethanol tolerance among the cell types. Ethanol seemed to kill cells in the cell culture effectively in much lower concentrations than those currently used in tumour ablation.

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Citations

Aug 16, 2008·Journal of Experimental & Clinical Cancer Research : CR·Enrico M ZardiAntonella Afeltra
Oct 11, 2016·Oral Oncology·Miguel López-Lázaro
Aug 24, 2017·FEMS Microbiology Reviews·Doris VandeputteJeroen Raes
Dec 8, 2020·Frontiers in Chemistry·Pingping LiangMarcel P Bruchez
May 11, 2021·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Pintu-On Chantarawaratit, Tipanan Yanisarapan
Jul 25, 2021·Cancers·Francisco D Rodriguez, Rafael Coveñas

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