Toxicity of Fe2 O3 nanoparticles on human blood lymphocytes

Journal of Biochemical and Molecular Toxicology
Evelyn AssadianJalal Pourahmad

Abstract

Magnetic nanoparticles (NPs) are used to a large extent in the targeted delivery of therapeutic agents. In this study, we aimed to investigate the possible toxicity of Fe2 O 3 NPs on human cells, including blood lymphocytes. We isolated blood lymphocytes from healthy humans using Ficoll polysaccharide and subsequently by gradient centrifugation. Then, the toxicity parameters, including cell viability, reactive oxygen species (ROS) formation, lipid peroxidation, cellular glutathione (GSH) level, mitochondrial and lysosomal damage, were measured in blood lymphocytes after exposure to Fe 2 O 3 NPs. Our results indicated that Fe 2 O 3 NPs significantly (dependent on concentration) reduced the cell viability, and the IC 50 was determined to be 1 mM. With increasing concentrations, we found that Fe 2 O 3 NPs-induced cell toxicity was associated with a significant increase in intracellular ROS and loss of mitochondrial membrane potential and lysosomal membrane leakiness. Consequently, these NPs at different concentrations affect GSH level and cause oxidative stress in human lymphocytes.

References

Mar 30, 2004·The Oncologist·David S Goodsell
Feb 17, 2005·European Journal of Pharmacology·An-Chin ChengMin-Hsiung Pan
Jun 4, 2005·Cell Death and Differentiation·J C GoldsteinD R Green
Jul 6, 2010·European Journal of Pharmacology·Jalal PourahmadAli Reza Khajeamiri
Sep 13, 2013·BioMed Research International·Amruta MankeYon Rojanasakul
Mar 29, 2014·Journal of Food and Drug Analysis·Peter P FuHongtao Yu
Mar 29, 2014·Journal of Food and Drug Analysis·Haohao WuY Martin Lo
Oct 4, 2015·Redox Biology·Joe Dan DunnThierry Soldati
Mar 19, 2016·Environmental Toxicology·Kiruthika SundarrajEkambaram Perumal
Oct 30, 2016·Regulatory Toxicology and Pharmacology : RTP·Leila SadeghiVahid Yousefi Babadi
Jan 12, 2017·International Journal of Molecular Sciences·Ahmed Abdal DayemSsang-Goo Cho
Mar 23, 2017·Neurotoxicity Research·Vasanth DhakshinamoorthyEkambaram Perumal
May 26, 2015·Nanomaterials·Moataz MekawyTeiji Tominaga
Jan 26, 2018·Journal of Molecular Neuroscience : MN·Vijayprakash ManickamEkambaram Perumal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.