Toxicity of Flow Line, Durafill VS, and Dycal to dental pulp cells: effects of growth factors.

Journal of Endodontics
Alyssa FureyDoug Lobner

Abstract

The objective was to determine the effects of growth factor treatment on dental pulp cell sensitivity to toxicity of 2 composite restoration materials, Flow Line and Durafill VS, and a calcium hydroxide pulp capping material, Dycal. Toxicity of the dental materials to cultures of primary dental pulp cells was determined by the MTT metabolism assay. The ability of 6 different growth factors to influence the toxicity was tested. A 24-hour exposure to either Flow Line or Durafill VS caused approximately 40% cell death, whereas Dycal exposure caused approximately 80% cell death. The toxicity of Flow Line and Durafill VS was mediated by oxidative stress. Four of the growth factors tested (bone morphogenetic protein [BMP]-2, BMP-7, epidermal growth factor [EGF], and transforming growth factor [TGF]-beta) decreased the basal MTT values while making the cells resistant to Flow Line and Durafill VS toxicity except BMP-2, which made the cells more sensitive to Flow Line. Treatment with fibroblast growth factor-2 caused no change in basal MTT metabolism, prevented the toxicity of Durafill VS, but increased the toxicity of Flow Line. Treatment with insulin-like growth factor-I (IGF-I) increased basal MTT metabolism and made the cells resis...Continue Reading

Citations

Apr 4, 2012·International Endodontic Journal·M G GandolfiC Prati
Nov 28, 2013·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Khalid Al-HezaimiFranklin Tay
Mar 22, 2014·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Nawaf LabbanJeffrey A Platt
Jun 25, 2014·TheScientificWorldJournal·Claudio PoggioLivia Visai
Nov 2, 2013·The Journal of Oral Implantology·Rosa-María Díaz-SánchezDaniel Torres-Lagares
May 8, 2016·Clinical Oral Investigations·Xin LiBart Van Meerbeek
Aug 20, 2015·Journal of Dental Research·A N MooreJ S Colombo
Nov 10, 2020·BioMed Research International·Paras AhmadMartin J Stoddart
Aug 28, 2021·Journal of Pharmacy & Bioallied Sciences·Muqthadir Siddiqui Mohammed AbdulShalini Singh
Aug 28, 2021·Journal of Pharmacy & Bioallied Sciences·Jenny AtomAbdulkarim S BinShaya

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