Toxicity phenotype does not correlate with phylogeny of Cylindrospermopsis raciborskii strains

Systematic and Applied Microbiology
Karina StuckenM Vásquez

Abstract

Cylindrospermopsis raciborskii is a species of freshwater, bloom-forming cyanobacterium. C. raciborskii produces toxins, including cylindrospermopsin (hepatotoxin) and saxitoxin (neurotoxin), although non toxin-producing strains are also observed. In spite of differences in toxicity, C. raciborskii strains comprise a monophyletic group, based upon 16S rRNA gene sequence identities (greater than 99%). We performed phylogenetic analyses; 16S rRNA gene and 16S-23S rRNA gene internally transcribed spacer (ITS-1) sequence comparisons, and genomic DNA restriction fragment length polymorphism (RFLP), resolved by pulsed-field gel electrophoresis (PFGE), of strains of C. raciborskii, obtained mainly from the Australian phylogeographic cluster. Our results showed no correlation between toxic phenotype and phylogenetic association in the Australian strains. Analyses of the 16S rRNA gene and the respective ITS-1 sequences (long L, and short S) showed an independent evolution of each ribosomal operon. The genes putatively involved in the cylindrospermopsin biosynthetic pathway were present in one locus and only in the hepatotoxic strains, demonstrating a common genomic organization for these genes and the absence of mutated or inactivated b...Continue Reading

References

Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J LaneN R Pace
Nov 1, 1994·Molecular & General Genetics : MGG·S T LovettP T Drapkin
Jul 1, 1997·International Journal of Systematic Bacteriology·B A NeilanA E Goodman
Jan 27, 1999·Bioinformatics·D Posada, K A Crandall
Jul 22, 1999·Toxicon : Official Journal of the International Society on Toxinology·N LagosY Oshima
Mar 7, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·T KissL Vörös
Dec 21, 2002·Molecular Ecology·B A NeilanB P Burns
Jan 21, 2003·Journal of Bacteriology·Joel G HashimotoThomas M Schmidt
Feb 14, 2003·FEMS Microbiology Letters·Rosa AlduinaAnna M Puglia
Mar 14, 2003·Environmental Toxicology·Dilwyn J Griffiths, Martin L Saker
Sep 29, 2004·Toxicon : Official Journal of the International Society on Toxinology·Daniela CastroMónica Vásquez
Feb 5, 2005·Applied and Environmental Microbiology·Muriel GuggerJean-François Humbert
Sep 3, 2005·Nature Reviews. Microbiology·Laura S FrostAriane Toussaint
Mar 2, 2006·Journal of Molecular Evolution·Ralf KellmannBrett A Neilan
Nov 17, 2007·Nature Protocols·Brant J Bassam, Peter M Gresshoff
Dec 11, 2007·Applied and Environmental Microbiology·Troco Kaan MihaliBrett A Neilan
Mar 30, 2016·Applied Microbiology and Biotechnology·Zhanfei HeBaolan Hu

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Citations

Aug 3, 2014·Applied Microbiology and Biotechnology·Cristiana MoreiraVitor Vasconcelos
Nov 16, 2010·Molecular Biology and Evolution·Shauna A MurrayBrett A Neilan
Feb 22, 2011·Toxicon : Official Journal of the International Society on Toxinology·Otilia AntalAgnes Vehovszky
Aug 10, 2010·Toxicon : Official Journal of the International Society on Toxinology·Katia Soto-LiebeMónica Vásquez
Nov 6, 2013·Marine Drugs·Cristiana MoreiraAgostinho Antunes
Sep 24, 2013·Environmental Science. Processes & Impacts·Armah A de la CruzDionysios D Dionysiou
Jul 4, 2017·Frontiers in Microbiology·Pablo GuimarãesLucas J Stal

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