Toxicity Threshold of Perfluorocarbon Liquids for Intraocular Use: Dose-Response Assessment of In Vitro Cytotoxicity of Possible Contaminants.

Translational Vision Science & Technology
Mario R RomanoJana D'Amato Tóthová

Abstract

This study assessed the cytotoxicity of the impurities detected in the perfluorooctane (PFO) batches for vitreoretinal surgery that were associated with serious adverse incidents of ocular toxicity, namely, the perfluorooctanoic acid (PFOA), 1H,1H,7H-dodecafluoro-1-heptanol (DFH), 1H-perfluorooctane (1H-PFO), ethylbenzene, anhydrous p-xylene, and perfluoro-2-butyltetrahydrofurane, and two additional substances 1H,1H,1H,2H,2H-perfluorooctane (5H-PFO) and hexafluoro-1,2,3,4-tetrachlorobutane. Serial dilutions were tested by in vitro direct contact cytotoxicity test, validated in accordance with the ISO 10993-5:2009 standard using BALB3T3 and ARPE-19 cell lines, after sample application for 24 hours. Six of the eight tested substances were cytotoxic according to the above-mentioned ISO standard. Anhydrous p-xylene, ethylbenzene, and PFOA were the most cytotoxic impurities as traces 1.55 ppm, 1.06 ppm, and 28.4 ppm reached the cytotoxicity limit, respectively. Hexafluoro-1,2,3,4-tetrachlorobutane, DFH, and 1H-PFO were cytotoxic at 980, 22,500, and 123,000 ppm, respectively. Both 5H-PFO and perfluoro-2-butyltetrahydrofuran were non-cytotoxic at the highest available concentrations (≥970,000 ppm). The dose-response curves allowed to ...Continue Reading

References

Mar 1, 1995·Survey of Ophthalmology·G A PeymanB Sullivan
May 1, 1996·Archives of Ophthalmology·R D BourkeJ R Sparrow
May 26, 2017·Retina·J Carlos PastorGirish K Srivastava
May 26, 2017·Retina·Stanley Chang, R Nigel Simpson
Jan 25, 2018·Scientific Reports·Girish K SrivastavaJ Carlos Pastor
Oct 23, 2018·Investigative Ophthalmology & Visual Science·Dirk-Henning MenzHans Hoerauf
Jun 7, 2019·Translational Vision Science & Technology·Dirk-Henning MenzHans Hoerauf
Nov 17, 2019·Regulatory Toxicology and Pharmacology : RTP·Girish K SrivastavaJ Carlos Pastor

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