PMID: 9443375Aug 1, 1997Paper

Toxicological and molecular characterization of pyrethroid-resistant horn flies, Haematobia irritans: identification of kdr and super-kdr point mutations

Insect Biochemistry and Molecular Biology
F D GuerreroS E Kunz

Abstract

Two pyrethroid-resistant strains of horn flies were found to be 17- and 688-fold more resistant to permethrin and 17- and 11,300-fold more resistant to cyhalothrin than a susceptible control strain. Synergism experiments with piperonyl butoxide showed that both target site insensitivity and metabolic resistance mechanisms were present in the Super Resistant strain. Using the reverse transcriptase-polymerase chain reaction (RT-PCR), a 0.9 kb fragment of the putative sodium channel gene from susceptible and resistant flies was cloned and sequenced. Two sequence variants were detected, presumably arising from alternative splicing of transcripts. The amino acid sequences deduced from the resistant and susceptible fly gene fragments were identical except for three amino acid substitutions, two of which have been associated with resistance in house flies. A leucine to phenylalanine substitution associated with knockdown resistance (kdr) was found in both resistant strains. A methionine to threonine substitution associated with super-kdr was found in the Super Resistant strain. Translation of poly(A)+ RNA followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) detected translation products whose concentrations...Continue Reading

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Citations

Jan 9, 2007·Invertebrate Neuroscience : in·Ke Dong
Jul 14, 2005·Genetica·Lilian Castiglioni, Hermione Elly Melara de Campos Bicudo
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May 17, 2006·Biochemical and Biophysical Research Communications·Qiang XuNannan Liu
Apr 12, 2003·Medical and Veterinary Entomology·C M MontagnaA M Pechen de d-Angelo

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