Toxicological assessment of a prototype e-cigaret device and three flavor formulations: a 90-day inhalation study in rats

Inhalation Toxicology
Michael S WerleyWillie J Mckinney

Abstract

A prototype electronic cigaret device and three formulations were evaluated in a 90-day rat inhalation study followed by a 42-day recovery period. Animals were randomly assigned to groups for exposure to low-, mid- and high-dose levels of aerosols composed of vehicle (glycerin and propylene glycol mixture); vehicle and 2.0% nicotine; or vehicle, 2.0% nicotine and flavor mixture. Daily targeted aerosol total particulate matter (TPM) doses of 3.2, 9.6 and 32.0 mg/kg/day were achieved by exposure to 1 mg/L aerosol for 16, 48 and 160 min, respectively. Pre-study evaluations included indirect ophthalmoscopy, virology and bacteriological screening. Body weights, clinical observations and food consumption were monitored weekly. Plasma nicotine and cotinine and carboxyhemoglobin levels were measured at days 28 and 90. After days 28, 56 and 90, lung function measurements were obtained. Biological endpoints after 90-day exposure and 42-day recovery period included clinical pathology, urinalysis, bronchoalveolar fluid (BALF) analysis, necropsy and histopathology. Treatment-related effects following 90 days of exposure included changes in body weight, food consumption and respiratory rate. Dose-related decreases in thymus and spleen weight...Continue Reading

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Citations

Jul 1, 2017·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·A Verhaegen, L Van Gaal
Jun 12, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Elizabeth M MartinIlona Jaspers
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Sep 27, 2019·Inhalation Toxicology·Guanghe WangWeimin Song
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Methods Mentioned

BETA
bronchoalveolar lavage
lavage
bronchoalveolar

Software Mentioned

SAS
BioSTAT

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