Toxicological evaluation of harmful substances by in situ enzymatic and biological detection in high-performance thin-layer chromatography

Journal of Chromatography. a
C Weins, H Jork

Abstract

The efficiency of a chromatographic analysis method is determined by the selectivity of the chromatographic separation and the specificity of the detection method. In the case of high-performance thin-layer chromatography (HPTLC) the separated components can be detected and quantified directly on the chromatogram by physical and chemical methods. By coupling high-performance thin-layer chromatography with biological or biochemical inhibition tests it was possible to detect toxcologically active substances in situ. A linear relationship was shown between the signal of the inhibition of cholinesterase and the concentration of the inhibitor using a constant enzyme concentration and a constant incubation time. The graph of the inhibition of the luminescence of Photobacterium vibrio fisheri in relation to the concentration of pentachlorophenol (range 20-80 ng) is nearly linear. Measurements were done by using a densitometer or a videodensitometric scanner.

Citations

Jan 18, 2011·Journal of Chromatography. a·A Marston
Oct 4, 2005·Journal of Chromatography. a·Susanne FabelMichael G Weller
Feb 2, 2017·Phytochemical Analysis : PCA·Sarah Bräm, Evelyn Wolfram
Aug 4, 2020·Critical Reviews in Analytical Chemistry·Barbora LegerskáStanislav Miertuš

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