Toxigenic and non-toxigenic patterns I, II and III and biofilm-forming ability in Bacteroides fragilis strains isolated from patients diagnosed with colorectal cancer

Gut Pathogens
Seyedesomaye JasemiMohammad Mehdi Feizabadi

Abstract

Enterotoxigenic Bacteroides fragilis (ETBF) associated with the initiation and progression of colorectal cancer (CRC) has been alarmingly reported all over the world. In this study, simultaneous investigation of toxigenic and non-toxigenic patterns I, II and III and biofilm formation ability of Bacteroides fragilis isolated from patients with colorectal cancer was performed. Thirty-one patients diagnosed with CRC and thirty-one control subjects were recruited in this study. Specimens were cultured on BBE and BBA culture media. Classical phenotypic identification tests and PCR was performed to verify Bacteroides fragilis presence. Also, biofilm-forming ability and expression of bft gene were assessed under biofilm and planktonic forms. A total of 68 B.fragilis was isolated from all colorectal tissue, of which 13 isolates (19.1%) (11 isolates from CRC and 2 from normal tissue) were positive for bft gene. The abundance patterns of I, II and III were as follow in descending order; pattern I > pattern III > pattern II in CRC subjects and pattern II > pattern III > pattern I in normal tissues. Also, pattern I showed higher biofilm formation ability compared to other patterns. Toxin expression was significantly reduced in biofilm form...Continue Reading

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Citations

Dec 18, 2020·Frontiers in Cellular and Infection Microbiology·Xue FengQiling Li
Apr 25, 2021·Annals of Clinical Microbiology and Antimicrobials·Seyedesomaye JasemiMohammad Mehdi Feizabadi

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Datasets Mentioned

BETA
MN955555.1
MN955554.1
MN950426.1
MN937242.1
MK792343.1

Methods Mentioned

BETA
biopsy
PCR
ELISA

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