PMID: 44443Nov 1, 1979

Toxin production by Clostridium botulinum in grass

Applied and Environmental Microbiology
S NotermansM van Schothorst

Abstract

Investigations on farms where botulism has occurred in cows showed that proteolytic Clostridium botulinum type B was present in newly made grass silages. Experiments were undertaken to study growth and toxin production of C. botulinum in grass. Of the strains tested only proteolytic strains of C. botulinum types A and B were able to produce toxin with grass as a substrate. Proteolytic strains of type B produced both medium (12S) and large (16S) toxin forms. The minimal water activity (aw) for toxin production at pH 6.5 and 5.8 was 0.94. At pH 5.3, toxin was produced at an aw of 0.985. These results indicate that proteolytic strains of C. botulinum (if present) may multiply and produce toxin in wilted grass silages.

References

Jul 9, 2008·Epidemiology and Infection·J MyllykoskiHannu Korkeala
Jan 8, 2020·Toxins·Christine Rasetti-EscargueilMichel R Popoff
Mar 20, 2010·Critical Reviews in Food Science and Nutrition·Miia LindströmHannu Korkeala
Feb 1, 1990·The Journal of Applied Bacteriology·M K Woolford
Sep 14, 2019·Journal of Applied Microbiology·C L S Ávila, B F Carvalho

Related Concepts

Botulism
Botulinum Toxins
Alopecurus
Spores, Bacterial
Silage
Hydrogen-Ion Concentration
Animal Feed (Substance)
Clostridium botulinum

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