Toxoplasma gondii and Neospora caninum in Tolai Hares (Lepus tolai) Intended for Human Consumption in China: Seroprevalence, DNA Detection, and Genotyping

Foodborne Pathogens and Disease
Wei CongAi-Dong Qian

Abstract

Currently, there is no information available on the detection of Toxoplasma gondii and Neospora caninum in the tissues of Tolai hares in China. This study aimed to investigate the prevalence of these protozoan parasites in Tolai hares obtained from Shandong province, eastern China, between January 2016 and June 2017. Serum and brain tissue samples of 358 Tolai hares were obtained and detected for the presence of antibody and parasite DNAs by serodiagnosis and polymerase chain reaction (PCR), respectively. The seroprevalence of T. gondii and N. caninum infection in Tolai hares was 8.10% (29/358) and 0.84% (3/358), respectively. However, all the 358 tested Tolai hares were negative for N. caninum by PCR and T. gondii DNA was detected in 23 Tolai hares (6.42%, 23/358). The positive T. gondii DNA was genotyped at 11 genetic markers using multilocus PCR-restriction fragment length polymorphism technology. Of the 23 positive samples, only 2 of them produced complete genotyping results, and were identified as ToxoDB Genotype #9. This is the first report to detect T. gondii in the tissues of Tolai hares from China and the first study to focus on N. caninum in Tolai hares from China.

References

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Jan 4, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Wei CongXing-Quan Zhu
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Aug 27, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yang ZouWei Cong

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Citations

Jun 15, 2019·Parasite : Journal De La Société Française De Parasitologie·Weifeng QianTianqi Wang
Nov 6, 2020·Research in Veterinary Science·S Almeria, J P Dubey

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
genotyping

Software Mentioned

SAS Statistical Analysis System

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