Toxoplasma gondii Arginine Methyltransferase 1 (PRMT1) Is Necessary for Centrosome Dynamics during Tachyzoite Cell Division

MBio
Kamal El BissatiKami Kim

Abstract

The arginine methyltransferase family (PRMT) has been implicated in a variety of cellular processes, including signal transduction, epigenetic regulation, and DNA repair pathways. PRMT1 is thought to be responsible for the majority of PRMT activity in Toxoplasma gondii, but its exact function is unknown. To further define the biological function of the PRMT family, we generated T. gondii mutants lacking PRMT1 (Δprmt1) by deletion of the PRMT1 gene. Δprmt1 parasites exhibit morphological defects during cell division and grow slowly, and this phenotype reverses in the Δprmt::PRMT1mRFP complemented strain. Tagged PRMT1 localizes primarily in the cytoplasm with enrichment at the pericentriolar material, and the strain lacking PRMT1 is unable to segregate progeny accurately. Unlike wild-type and complemented parasites, Δprmt1 parasites have abnormal daughter buds, perturbed centrosome stoichiometry, and loss of synchronous replication. Whole-genome expression profiling demonstrated differences in expression of cell-cycle-regulated genes in the Δprmt1 strain relative to the complemented Δprmt1::PRMT1mRFP and parental wild-type strains, but these changes do not correlate with a specific block in cell cycle. Although PRMT1's primary bi...Continue Reading

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Citations

Oct 4, 2016·Cellular Microbiology·Allan L ChenPeter J Bradley
Dec 28, 2016·Cytoskeleton·Juliette Morlon-GuyotWassim Daher
Jun 23, 2018·Annual Review of Microbiology·Kami Kim
Oct 20, 2019·Cellular Microbiology·Ksenia SemenovskayaSébastien Besteiro

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Datasets Mentioned

BETA
GSE73177

Methods Mentioned

BETA
transgenic
PCR
fluorescence microscopy
electrophoresis
transmission electron microscopy
transfection
pulldown
transfections
scraping
chip

Software Mentioned

Biosystems 4000 series Explorer
MASCOT
Adobe Photoshop CS
GeneSpring GX11
GSEA
GeneSpring
Applied

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