Toxoplasma gondii: enzyme-linked immunosorbent assay using different fragments of recombinant microneme protein 1 (MIC1) for detection of immunoglobulin G antibodies

Experimental Parasitology
Lucyna HolecJózef Kur

Abstract

Three different fragments of microneme 1 protein termed, r-MIC1ex2, r-MIC1ex34 and r-MIC1 of Toxoplasma gondii, were expressed in Escherichia coli as fusion proteins containing six histidyl residues at N- and C-terminal. After purification by metal affinity chromatography, these recombinant proteins were tested for their usefulness as antigens in an enzyme-linked immunosorbent assay for the detection of immunoglobulin G. Ninety-eight sera from patients with different stages of invasion and 24 sera from seronegative patients were examined. There was no significant difference observed in the antigenicity for human serum samples from patients with acute toxoplasmosis between three recombinant types of MIC1 antigen (96.1% for r-MIC1ex2 antigen and 100% for both r-MIC1ex34 and r-MIC1 proteins). Sera from chronic infections (with low titers of IgG antibody) showed significant lower sensitivity, especially for r-MIC1ex34 and r-MIC1 antigens (75%, 52.7% and 36.1% for r-MIC1ex2, r-MIC1ex34 and r-MIC1, respectively). These results indicate that the strongest antigenic region of the MIC1 is encoding by the second exon of mic1 gene. When r-MIC1ex2 (N-terminal fragment of protein) was combined with MAG1 (matrix antigen) and MIC3 (microneme ...Continue Reading

References

Jan 31, 1998·Molecular Microbiology·J M DobrowolskiL D Sibley
Mar 3, 1998·Trends in Microbiology·J F Dubremetz
Sep 2, 1998·International Journal for Parasitology·J P Dubey
Jul 12, 2001·Glycobiology·E V LourençoA Panunto-Castelo
Jan 3, 2003·Protein Expression and Purification·Elzbieta Hiszczyńska-SawickaJózef Kur
Feb 3, 2005·The Journal of Experimental Medicine·Odile CérèdeMaryse Lebrun
Apr 18, 2006·Microbes and Infection·Elaine V LourençoMaria-Cristina Roque-Barreira
Jan 5, 2007·Clinical and Vaccine Immunology : CVI·Lucyna HolecJózef Kur

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Citations

Oct 5, 2012·Clinical and Vaccine Immunology : CVI·Lucyna Holec-GasiorDorota Drapala
Jan 6, 2012·Clinical and Vaccine Immunology : CVI·Jianfang DaiJin Si
Dec 10, 2009·Future Microbiology·Vera Lucia Pereira-ChioccolaChunlei Su
Jul 6, 2014·BioMed Research International·Xiao Teng ChingHemah Andiappan
Feb 24, 2015·Diagnostic Microbiology and Infectious Disease·Dorota DrapałaJózef Kur
Nov 24, 2016·Experimental Parasitology·Abdelbaset Eweda AbdelbasetMakoto Igarashi
Apr 27, 2018·Biomarkers in Medicine·Juan Gabriel Costa, María Julia Vilariño
Jul 30, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Dupadahalli Kotresha, Rahmah Noordin
Jun 8, 2017·Memórias do Instituto Oswaldo Cruz·Priscila T BaschirottoLeonardo Foti
May 28, 2020·Frontiers in Cellular and Infection Microbiology·Rochelle Haidee D YbañezYoshifumi Nishikawa

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