Toxoplasma gondii in vegetables from fields and farm storage facilities in the Czech Republic

FEMS Microbiology Letters
Michal SlanyIva Slana

Abstract

Infection with Toxoplasma gondii has usually been connected with consumption of improperly treated meat. However, contaminated water and products of plant origin have emerged as new sources of infection in the last years. Here, 292 vegetable samples-carrot, cucumber and lettuce-obtained from nine farms in the Czech Republic were examined using triplex qPCR targeting two specific T. gondii sequences. Irrigation water and water used for washing of vegetables were also included. Overall, a positivity rate of 9.6% was found in vegetable. The concentration varied between 1.31 × 100 and 9.00 × 102 oocysts/g of sample. A significant difference was found between the positivity of vegetables collected directly from fields and from farm storage rooms (4.4-8.6% vs. 10%-24.1%, respectively). All samples of irrigation water and water used to rinse vegetables were negative. Genotyping based on RFLP analysis using seven markers revealed the exclusive presence of genotype II.

References

Aug 29, 2002·The Journal of Parasitology·Kalmia E KnielJ P Dubey
Nov 26, 2003·FEMS Microbiology Reviews·Aurélien Dumètre, Marie Laure Dardé
Jul 9, 2004·Applied and Environmental Microbiology·Isabelle VillenaJean-Michel Pinon
Dec 24, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A LassP Myjak
May 7, 2009·Parasitology Research·Bastiaan G Meerburg, Aize Kijlstra
Aug 19, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·L David SibleyBenjamin M Rosenthal
Jan 6, 2010·The Journal of Parasitology·David S Lindsay, J P Dubey
Sep 29, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A LassP Myjak
May 15, 2012·Applied and Environmental Microbiology·Maud LéluEmmanuelle Gilot-Fromont
Dec 4, 2012·International Journal for Parasitology·D E Hill, J P Dubey
Jul 16, 2013·Trends in Parasitology·E Fuller Torrey, Robert H Yolken
May 29, 2015·Parasites & Vectors·Quan LiuXing-Quan Zhu
Oct 22, 2016·International Journal of Food Microbiology·Michal SlanyAlena Lorencova
Jun 27, 2017·Food Microbiology·Tiziana CaradonnaAnnunziata Giangaspero

❮ Previous
Next ❯

Citations

Apr 10, 2020·Parasites & Vectors·Cláudia S MarquesJosé Manuel Correia da Costa
May 8, 2020·FEMS Microbiology Letters·Nicola HoldenJeri Barak
Jul 31, 2020·Parasites & Vectors·Junqiang LiLongxian Zhang
Nov 21, 2021·Acta Parasitologica·Salma BerrouchIsabelle Villena

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.