Toxoplasma gondii infection of fibroblasts causes the production of exosome-like vesicles containing a unique array of mRNA and miRNA transcripts compared to serum starvation

Journal of Extracellular Vesicles
Samuel M Pope, Cecilia Lässer

Abstract

Until recently thought to be of little significance unless occurring during pregnancy, Toxoplasma gondii infection of human hosts is now known to play a larger role in mental health and is a growing concern in the health care community. We sought to elucidate a possible mechanism by which Toxoplasma infection may cause some of the behavioural pathology now associated with infection. We hypothesized that exosomes may be playing a role. We utilized electron microscopy to detect the presence and size of extracellular vesicles in the supernatants of Toxoplasma-infected human foreskin fibroblasts (HFF). We then utilized microarray analysis to discern mRNA and miRNA content of the vesicles isolated from supernatants of Toxoplasma-infected (Toxo) and serum-starved (SS) HFF. We recovered extracellular vesicles with a size consistent with exosomes that we called exosome-like vesicles (ELVs) from the supernatants of SS and Toxo cultures. The mRNA and miRNA content of these ELVs was highly regulated creating specific and unique expression profiles comparing Toxo ELVs, SS ELVs and RNA isolated from whole cell homogenates. Interestingly, among the most enriched mRNA isolated from ELVs of Toxo cells are 4 specific mRNA species that have been...Continue Reading

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Citations

Oct 28, 2015·Expert Review of Molecular Diagnostics·Akira YokoiTakahiro Ochiya
Oct 5, 2015·Trends in Parasitology·Gillian CoakleyAmy H Buck
Jul 4, 2015·Genesis : the Journal of Genetics and Development·Antony M Jose
Feb 28, 2015·International Journal for Parasitology·Louis-Philippe LerouxFlorence S Dzierszinski
Sep 25, 2014·Frontiers in Immunology·Sergio MontanerAntonio Marcilla
Aug 29, 2014·PLoS Pathogens·Olivia Twu, Patricia J Johnson
May 22, 2016·Current Opinion in Microbiology·Matthias Marti, Patricia J Johnson
May 28, 2016·Nature Reviews. Endocrinology·Adeel SafdarMark A Tarnopolsky
Jun 28, 2016·Cancer Metastasis Reviews·Li MinZhenfeng Duan
Jul 6, 2018·Parasite Immunology·Valeria Oliveira SilvaVera Lucia Pereira-Chioccola
Nov 27, 2018·FEMS Microbiology Reviews·Sukhvinder GillPatrick Forterre
Oct 12, 2019·Proceedings of the National Academy of Sciences of the United States of America·Anand Kumar Rai, Patricia J Johnson
Mar 4, 2020·PloS One·Allecineia Bispo da CruzVera Lucia Pereira-Chioccola
Jul 26, 2018·Journal of Extracellular Vesicles·Rodrigo P SoaresAna Claudia Torrecilhas
Jun 4, 2019·Frontiers in Cellular and Infection Microbiology·Kayla L MenardEric Y Denkers
Nov 14, 2019·The Korean Journal of Parasitology·Wen WangJiaqing Zhao
Sep 15, 2020·Frontiers in Cellular and Infection Microbiology·Joshua Seun Olajide, Jianping Cai
May 12, 2017·Cold Spring Harbor Perspectives in Medicine·Adeel Safdar, Mark A Tarnopolsky
Jan 31, 2019·Frontiers in Immunology·Zhenyu WuXi Sun
Apr 9, 2020·Frontiers in Cellular and Infection Microbiology·André Cronemberger-AndradeAna Claudia Torrecilhas

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Methods Mentioned

BETA
electron microscopy
chip
PCR

Software Mentioned

GeneSpring ©

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