Toxoplasma gondii prevalence in food animals and rodents in different regions of China: isolation, genotyping and mouse pathogenicity

Parasites & Vectors
Lin WangJilong Shen

Abstract

Recent studies of Toxoplasma gondii isolates from animals in different regions of China have shown a limited genetic diversity and type China 1 was the dominant genotype of T. gondii prevalent in Chinese animals. However, little has been known concerning the isolation and genotyping of T. gondii circulating in chickens, pigs and rodents in China. The aim of the study was to characterize samples of T. gondii isolates obtained from naturally infected cats, pigs and free-range chickens slaughtered for human consumption in China. In the present study, brain tissues of 77 animals collected from different areas of China, including 24 free-range chickens (Gallus domesticus) , 13 voles (Rattus flavipectus), 23 pigs and 17 cats, were bioassayed in mice and viable T. gondii were isolated from the brains of eleven. These eleven T. gondii isolates were maintained in Kunming (KM) outbred mice and DNA isolated from tissues of infected mice was characterized using 11 PCR-restriction fragment length polymorphism (PCR-RFLP) markers: SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3. Moreover, to determine mouse virulence of China 1 lineage of parasites, a TgCtgy5 genotype isolate was selected randomly and assessed in KM mice...Continue Reading

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Citations

Jul 18, 2014·The Korean Journal of Parasitology·Maha H Elamin
Jun 9, 2016·BioMed Research International·Yongjie FengYurong Yang
Nov 22, 2017·Parasites & Vectors·Weisheng ChengJilong Shen
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Jun 12, 2021·Veterinary Research·Mercedes Fernández-EscobarLuis Miguel Ortega-Mora
Dec 4, 2020·Veterinary Parasitology·Jitender P DubeyChunlei Su

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Datasets Mentioned

BETA
JX218225
JX218226
JX218227

Methods Mentioned

BETA
genotyping
PCR
electrophoresis

Software Mentioned

Apico
BioEdit Sequence Align - ment Editor
SplitsTree4

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