PMID: 11906451Mar 22, 2002Paper

Toxoplasma gondii Rab5 enhances cholesterol acquisition from host cells

Cellular Microbiology
Bruno RobibaroKeith A Joiner

Abstract

The role of endocytosis in nutrient uptake by Toxoplasma gondii is unknown. To explore this issue, we characterized an endosomal compartment by identifying a T. gondii Rab5 homologue, a molecular marker for early endosomes in eukaryotic cells. The deduced amino acid sequence of the T. gondii Rab5 gene encodes a protein of 240 amino acids, which we termed TgRab51. TgRab51 was epitope-tagged at the N-terminus, expressed in the parasite, and localized by immunofluorescence and immunoelectron microscopy to tubulovesicular structures anterior to the parasite nucleus and adjacent to, but distinct from the Golgi. By immunofluorescence analysis, TgRab51wt-HA staining partially overlapped with Golgi/TGN markers, but not with the T. gondii secretory organelles. A dominant positive mutant, TgRab51Q103L-HA, enhanced uptake of exogenous cholesterol analogues in intracellular parasites, augmented formation of lipid droplets and accelerated parasite growth. Brefeldin A disrupted the TgRab51 compartment, and altered the distribution of fluorescent exogenous cholesterol in cells expressing TgRab51Q103L-HA. These results suggest that TgRab51 facilitates sterol uptake, possibly through a Golgi-dependent pathway.

References

Feb 1, 1989·Journal of Clinical Pathology·T G WreghittJ Wallwork
Mar 1, 1985·The Journal of Cell Biology·P GreenspanS D Fowler
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E R Pfefferkorn
Feb 1, 1982·Experimental Parasitology·J D Schwartzman, E R Pfefferkorn
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y T ChenH P Moore
Mar 10, 1995·The Journal of Biological Chemistry·S HoffenbergB F Dickey
Jan 1, 1994·Parasitology Research·B A NicholsC E Pavesio
Oct 1, 1993·Seminars in Cell Biology·L D Sibley
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R G Donald, D S Roos
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·J C SchwabK A Joiner
May 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A GallinoF Follath
Jan 1, 1997·Annual Review of Microbiology·A P Sinai, K A Joiner
Mar 14, 1998·Molecular Biology of the Cell·C Prescianotto-Baschong, H Riezman
Apr 4, 1998·Electrophoresis·B NagelkerkenP van der Sluijs
Apr 27, 1999·Biochimica Et Biophysica Acta·L Liscum, N J Munn
Sep 7, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A SingJ Heesemann
Jun 1, 1960·Experimental Parasitology·J D FULTON, D F SPOONER

❮ Previous
Next ❯

Citations

Mar 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Nitin PatelAmitabha Mukhopadhyay
Dec 6, 2005·BMC Biology·Jay R RadkeMichael W White
Dec 29, 2010·The Journal of Veterinary Medical Science·Yoshifumi NishikawaXuenan Xuan
Jul 25, 2009·International Journal for Parasitology·Jon P Boyle, Jay R Radke
May 13, 2005·Cellular Microbiology·Yoshifumi NishikawaIsabelle Coppens
Jun 10, 2004·Molecular Microbiology·Huân M NgôKeith A Joiner
Mar 12, 2008·Traffic·Lilach Sheiner, Dominique Soldati-Favre
Feb 11, 2011·Molecular Microbiology·Patrick A Vigueira, Kimberly S Paul
Oct 27, 2004·Molecular and Biochemical Parasitology·Friederike QuittnatIsabelle Coppens
Mar 2, 2005·Biochemical and Biophysical Research Communications·Júlia Rolão AraripeEdson Rondinelli
Nov 23, 2005·Trends in Parasitology·Sabrina Sonda, Adrian B Hehl
May 12, 2009·Memórias do Instituto Oswaldo Cruz·Markus Meissner, Katrin Klaus
Jul 17, 2014·MBio·Zhicheng DouVern B Carruthers
Jun 15, 2014·Journal of Cell Science·Dinkorma T Ouologuem, David S Roos
May 16, 2002·The Journal of Cell Biology·Keith A Joiner, David S Roos
Nov 26, 2002·The Journal of Biological Chemistry·Huân M NgôKeith A Joiner
Aug 23, 2019·Journal of Molecular Recognition : JMR·Vijay Kumar SrivastavaAnupam Jyoti
May 12, 2005·Médecine sciences : M/S·Françoise Baunaure, Gordon Langsley
Jun 8, 2018·Frontiers in Immunology·Adriana Lima VallochiPatricia T Bozza
Jul 15, 2004·Journal of Cell Science·Mei YangKeith A Joiner
Apr 25, 2003·Journal of Cell Science·Valerian NakaarKeith A Joiner
Sep 19, 2003·Journal of Medicinal Chemistry·Jerald C HinshawGlenn D Prestwich

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.