PMID: 30141842Dec 1, 2016Paper

Toxoplasma gondii Rhoptry Protein 17 Inhibits the Apoptosis of Mouse Macrophages via Activation of Activator Protein 1 Signaling

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Hai-long WangGuo-rong Yin

Abstract

To investigate the effect of Toxoplasma gondii rhoptry protein 17(ROP17) on γ-interferon (IFN-γ)-induced apoptosis of mouse J774A.1 monocyte macrophages. The J774A.1 cells were transfected with recombinant plasmid p3×Flag-CMV-14/TgROP17 or empty plasmid p3×Flag-CMV-14. After addition of IFN-γ, flow cytometry and Western blotting were performed to detect apoptosis and the protein levels of phosphorylated c-Jun and apoptosis-related proteins cleaved Caspase-3, Bcl-2, Bcl-xL and Bcl-3. The p3×Flag-CMV-14/TgROP17 plasmid and c-Jun shRNA were co-transfected into J774A.1 cells, after which IFN-γ was added to induce cell apoptosis. The levels of cleaved Caspase-3 and Bcl-3 were analyzed using Western blotting. Flow cytometry showed that the apoptosis rate of cells overexpressing ROP17[(3.73±0.51)%] was significantly lower than that of the control cells[(7.78±1.10)%, P<0.05]. Western blotting showed significant differences in protein levels of phosphorylated c-Jun(0.196±0.028 vs. 0.075±0.010), Bcl-3(0.461±0.063 vs. 0.108±0.013) and cleaved Caspase 3(0.015±0.004 vs. 0.174±0.026) between the cells overexpressing ROP17 and control cells (all P<0.05). However, the levels of Bcl-2 and Bcl-xL were not significantly different between the cell...Continue Reading

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Related Papers

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Hai-long WangGuo-rong Yin
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Linyu ZhouZhijun Bao
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Ying WuRui Ling
Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Chunming JiangZhenyu Bian
Asian Pacific Journal of Cancer Prevention : APJCP
Hong-Feng ZhangYun-Ming Su
© 2022 Meta ULC. All rights reserved