Toxoplasma gondii Rhoptry Protein ROP16 Mediates Partially SH-SY5Y Cells Apoptosis and Cell Cycle Arrest by Directing Ser15/37 Phosphorylation of p53

International Journal of Biological Sciences
Shuang ChangZuohua Mao

Abstract

Toxoplasma rhoptries, an unusual set of apical organelles that are associated with Toxoplasma infection may cause subversion of the host cell functions. Parasite rhoptry protein 16 (ROP16) is a regulator of host cell transcription during cell invasion in which it migrates into the host cell cytoplasm and subsequently localizes to the nucleus. In the present study, we found that overexpression of ROP16 could partially mediate human neuroblastoma SH-SY5Y apoptosis (12.47%) and cell cycle arrest in G1 phase (60.77%) in a p53 dependent manner by influencing the expression of Bax/Bcl-2 and p21/CDKs. ROP16 was identified to co-localize with p53, a novel direct interaction partner in the nucleus of SH-SY5Y. Furthermore, SH-SY5Y apoptosis via the mitochondria-dependent p53 pathway and cell cycle arrest caused by ROP16 dealt with direct serine 15/37 phosphorylation of p53. Our studies provide a new mechanism by which ROP16 interacts with the nucleus proteins which subsequently subverts the host cells functions.

Citations

Feb 9, 2017·Frontiers in Microbiology·Jin-Lei WangSi-Yang Huang
Apr 4, 2019·Cellular Microbiology·Mamadou Amadou DialloAnne Silvestre
Jun 25, 2020·Biomolecular NMR Assignments·Meng-Hsuan LinChun-Hua Hsu
May 14, 2019·Frontiers in Microbiology·Wanbo ZhuLi Yu
Dec 13, 2019·Frontiers in Cellular and Infection Microbiology·Paloma de Carvalho VieiraDaniel Adesse

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Methods Mentioned

BETA
nuclear translocation
scraping
electrophoresis
flow cytometry
immunoprecipitation
transfection
PCR
chip
co-immunoprecipitation
acetylation

Software Mentioned

Cell Quest
ModFit LT
GeneSpring GX
ABI Prism 7300
MASCOT

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