Toxoplasma gondii serine-protease inhibitor-1: A new adjuvant candidate for asthma therapy

PloS One
Ariadna S SotoAlejandra Goldman

Abstract

Serine-proteases are important players in the pathogenesis of asthma, promoting inflammation and tissue remodeling. It's also known that many serine protease inhibitors display immunomodulatory properties. TgPI-1 is a Toxoplasma gondii protein that exhibits broad spectrum inhibitory activity against serine proteases. In view of the increased prevalence of atopic disorders and the need to develop new treatment strategies we sought to investigate the potential of TgPI-1 for treating respiratory allergies. For this purpose, we developed a therapeutic experimental model. BALB/c mice were rendered allergic by intraperitoneal ovalbumin-alum sensitization and airway-challenged. Once the asthmatic phenotype was achieved, mice were intranasally treated with rTgPI-1 alone or with a mixture of rTgPI-1 and ovalbumin (OVA). A week later mice were given a secondary aerosol challenge. Treatment with rTgPI-1 alone or co-administered with OVA diminished bronchoalveolar eosinophilia, mucus production and peribronchial lung infiltration. This effect was accompanied by a lung resistance reduction of 26.3% and 50.3% respectively. Both treatments resulted in the production of lower levels of IL-4, IL-5, IFN-γ and regulatory IL-10 by thoracic lymph n...Continue Reading

References

Oct 1, 1990·The Journal of Allergy and Clinical Immunology·L B Schwartz
Apr 1, 1995·Respiration Physiology·K FujimotoK Sugane
Apr 26, 2000·Molecular and Biochemical Parasitology·V PszennyE J Bontempi
Jul 13, 2001·Pulmonary Pharmacology & Therapeutics·T M Cocks, J D Moffatt
Nov 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S MiikeH Kita
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Se-Woong OhWilliam R Henderson
May 30, 2002·Molecular and Biochemical Parasitology·Viviana PszennySergio O Angel
Sep 14, 2002·The Journal of Biological Chemistry·Meredith Teilhet MorrisVern B Carruthers
Nov 21, 2002·Pharmacology & Therapeutics·Heike SchäckeKhusru Asadullah
Jun 23, 2004·The Journal of Allergy and Clinical Immunology·F Estelle R SimonsKent T HayGlass
Nov 13, 2004·The Journal of Allergy and Clinical Immunology·Charles E Reed, Hirohito Kita
May 20, 2005·American Journal of Respiratory and Critical Care Medicine·Jodie L SimpsonPeter G Gibson
Apr 14, 2006·Current Topics in Medicinal Chemistry·Caroline GuayGuy M Tremblay
Aug 7, 2008·Chest·Ian M Adcock, Peter J Barnes
Oct 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Eli C LewisCharles A Dinarello
Oct 22, 2008·International Journal for Parasitology·Angelika WagnerUrsula Wiedermann
Nov 27, 2008·Clinical and Experimental Immunology·I FenoyA Goldman
Apr 23, 2010·Clinical and Experimental Immunology·E Maggi
May 29, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eyal OzeriEli C Lewis
Feb 9, 2013·Molecular and Cellular Biochemistry·Jia ZhouXiangdong Zhou
Aug 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Danny JonigkSabina Janciauskiene
Oct 25, 2013·Allergology International : Official Journal of the Japanese Society of Allergology·Marek JutelCezmi A Akdis
May 30, 2015·The World Allergy Organization Journal·Cezmi A Akdis, Mübeccel Akdis
Mar 1, 2016·Allergy, Asthma & Immunology Research·Marek JutelSylwia Smolinska

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Citations

Sep 7, 2018·Parasite Immunology·Candace M de AndradeCamila A Figueiredo
Nov 7, 2019·Pharmacology Research & Perspectives·Carlos D ZappiaFederico Monczor
Nov 19, 2019·Journal of Dermatological Science·Matías Damián Perrone SibiliaAlejandra Goldman

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Methods Mentioned

BETA
electrophoresis
blood collection
ELISA
flow cytometry

Software Mentioned

FlowJo

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