PMID: 8605904Oct 1, 1995Paper

Toxoplasma gondii surface antigen-1 in sera of HIV-infected patients as an indicator of reactivated toxoplasmosis

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
M F LetilloisB F Chumpitazi

Abstract

The major surface antigen from the proliferative form of Toxoplasma gondii (P-30 of SAG-1) was chosen as a target for exploration of Toxoplasma gondii reactivation in sera from immunocompromised patients. Samples were obtained from 37 HIV-infected subjects with lymphocyte levels of CD4+ < 200/mm3. The prevalence of IgG antibodies to Toxoplasma gondii was 64.9%. Ten patients had clinical symptoms of reactivated toxoplasmosis; eight of these had Toxoplasma encephalitis. The SAG-1 epitopes were found as circulating antigen in five cases with an immunocapture enzyme immunoassay (EIA). The EIA was improved with an IgG1 monoclonal antibody to SAG-1 and a streptavidinbiotin amplification. The sensitivity, specificity and positive predictive value were 30, 92 and 60%, respectively. The SAG-1 levels were compared with different biological parameters such as HIV p24 antigen, beta 2 microglobulin, CD4+ cell count and IgG antibodies to Toxoplasma gondii. The levels of SAG-1 in these patients were significantly higher than those in the 75 healthy control persons with or without a chronic Toxoplasma gondii infection. Therefore, SAG-1 may be involved as a marker of reactivated toxoplasmosis in HIV-infected patients.

References

Jul 1, 1975·The Journal of Infectious Diseases·R E Raizman, F A Neva
Feb 1, 1992·Experimental Parasitology·J Grimwood, J E Smith
Jan 1, 1991·Experimental Parasitology·R McLeodC Brown
Nov 1, 1988·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·A HasslH Flamm
Jan 1, 1988·The Journal of Infectious Diseases·B J Luft, J S Remington
Nov 1, 1988·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·A HasslH Flamm
Aug 1, 1985·Annals of Internal Medicine·D H SheppJ D Meyers
Jun 1, 1995·Journal of Clinical Microbiology·B F ChumpitaziA Goullier-Fleuret
Nov 1, 1993·European Journal of Epidemiology·H J StellbrinkS Fenske
Jul 1, 1993·Journal of Clinical Microbiology·J Dupouy-CametF Derouin

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Citations

Sep 22, 2001·International Journal for Parasitology·C G Lüder, F Seeber
Jul 16, 2002·Memórias do Instituto Oswaldo Cruz·Marcelo Simão Ferreira, Aércio Sebastião Borges
Mar 1, 1997·The Korean Journal of Parasitology·D W ShinT J Rho
Oct 23, 2009·Journal of Perinatology : Official Journal of the California Perinatal Association·B Shouman, R Badr

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