Abstract
Toxoplasma gondii atypical type II genotype was diagnosed in a pet peach-faced lovebird (Agapornis roseicollis) based on histopathology, immunohistochemistry, and multilocus DNA typing. The bird presented with severe neurological signs, and hematology was suggestive of chronic granulomatous disease. Gross post-mortem examination revealed cerebral hemorrhage, splenomegaly, hepatitis, and thickening of the right ventricular free wall. Histologic sections of the most significant lesions in the brain revealed intralesional protozoan organisms associated with malacia, spongiform changes, and a mild histiocytic response, indicative of diffuse, non-suppurative encephalitis. Immunohistochemistry confirmed the causative organisms to be T. gondii. DNA isolated from the brain was used to confirm the presence of T. gondii DNA. Multilocus genotyping based on SAG1, altSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico markers demonstrated the presence of ToxoDB PCR-RFLP genotype #3 and B1 gene as atypical T. gondii type II. The atypical type II strain has been previously documented in Australian wildlife, indicating an environmental transmission route.
References
Apr 1, 1991·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·W J Hartley, J P Dubey
Oct 6, 2001·Science·M E GriggJ C Boothroyd
May 29, 2002·Veterinary Parasitology·J P Dubey
Oct 29, 2003·Veterinary Parasitology·V KajerováK Sedlák
Aug 17, 2004·Journal of Virology·Livio HeathAnna-Lise Williamson
Nov 26, 2004·The Journal of Parasitology·J P DubeyT Lehmann
Dec 7, 2005·Molecular and Cellular Probes·Ralf EhrichtKonrad Sachse
Jun 26, 2008·The Journal of Parasitology·J HartleyJ P Dubey
Sep 26, 2008·Parasite : Journal De La Société Française De Parasitologie·M L Dardé
Aug 19, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·L David SibleyBenjamin M Rosenthal
Sep 22, 2009·Parasitology·C SuJ P Dubey
Mar 30, 2010·International Journal for Parasitology·N ParameswaranM E Grigg
Aug 2, 2011·International Journal for Parasitology·J P DubeyC Su
Oct 3, 2012·PloS One·Shuting PanAlan J Lymbery
Feb 13, 2013·Avian Diseases·Francisco Carlos FerreiraNelson Rodrigo da Silva Martins
Apr 3, 2014·Parasites & Vectors·Wei CongXing-Quan Zhu
Apr 25, 2014·Avian Diseases·Laryssa HoweWendi Roe
Aug 16, 2014·Veterinary Parasitology·Shannon L DonahoeJan Slapeta
Dec 3, 2014·Parasitology International·Shannon L DonahoeDavid N Phalen
Jun 24, 2015·International Journal for Parasitology. Parasites and Wildlife·Renata A CasagrandeDavid Driemeier