TP53 codon 72 polymorphism is associated with FGFR3 and RAS mutation in non-muscle-invasive bladder cancer

PloS One
Takashi KawaharaHiroyuki Nishiyama

Abstract

TP53, a well-known tumor-suppressor gene in bladder carcinogenesis, has a functional single-nucleotide polymorphism on codon 72. The aim of this study was to elucidate the association between TP53 codon 72 polymorphism and somatic mutations in bladder cancer. Germline TP53 codon 72 polymorphism and somatic mutations of 50 cancer-associated genes were analyzed in 103 bladder cancer patients (59 non-muscle-invasive and 44 muscle-invasive), using Taqman genotyping assay and target sequencing, respectively. The expression of FGF-FGFR signaling pathway genes was analyzed by RNA sequencing of frozen tissue. The allele frequency of TP53 codon 72 in our cohort was 37, 42, and 21% for Arg/Arg, Arg/Pro, and Pro/Pro, respectively. Interestingly, the prevalence of FGFR3 mutation was higher in patients with the Arg allele, whereas that of the RAS mutation was higher in patients without the Arg allele. The same association was seen in non-muscle-invasive bladder cancer (NMIBC) patients and no differences were observed in muscle-invasive bladder cancer patients. In NMIBC, FGFR1 expression was higher in patients without the Arg allele and FGFR3 expression was higher in patients with the Arg allele. The germline TP53 codon 72 polymorphism was a...Continue Reading

References

Jan 16, 1999·Molecular and Cellular Biology·M ThomasG Matlashewski
Mar 4, 2000·The Urologic Clinics of North America·A F Olumi
Mar 27, 2001·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·E Negri, C La Vecchia
Jun 8, 2001·The American Journal of Pathology·C BillereyF Radvanyi
Feb 5, 2003·Nature Genetics·Patrick DumontMaureen Murphy
Nov 26, 2003·International Journal of Cancer. Journal International Du Cancer·David Pim, Lawrence Banks
Mar 3, 2005·Journal of the National Cancer Institute·Hisayuki ShigematsuAdi F Gazdar
Apr 9, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yingchuan HuSteven A Ahrendt
Feb 8, 2006·Oncogene·M Siddique, K Sabapathy
Feb 28, 2006·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Amelia SzymanowskaJacek Jassem
Jun 10, 2006·Nature Clinical Practice. Urology·Claudio PelucchiCarlo La Vecchia
Jul 1, 2006·Science·Maria Teresa LandiBoris C Bastian
Sep 22, 2006·International Journal of Cancer. Journal International Du Cancer·Fiamma ButtittaAntonio Marchetti
Mar 28, 2008·The Journal of Investigative Dermatology·Maria Concetta FargnoliMaria T Landi
Mar 30, 2010·Nature Genetics·Lambertus A KiemeneyKari Stefansson
Aug 11, 2012·European Urology·Maximilian BurgerYair Lotan
Sep 28, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Snjezana DoganMarc Ladanyi
Jan 31, 2014·Nature·UNKNOWN Cancer Genome Atlas Research Network
Dec 24, 2014·Nature Reviews. Cancer·Margaret A Knowles, Carolyn D Hurst
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

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Citations

Sep 4, 2020·World Journal of Clinical Oncology·Kiran TripathiMinal Garg
Mar 30, 2021·Journal of Clinical Laboratory Analysis·Yihao LiaoNing Jiang

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Methods Mentioned

BETA
PCR
genotyping

Software Mentioned

Variant Caller
js
STAT view
CLC Genomics Workbench
Ion Ampli Seq Cancer Hotspot Panel
Sequence Detection
Torrent Suite
STAR

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