PMID: 9533755Apr 9, 1998Paper

TP53 genetic alterations in head-and-neck carcinomas from Brazil

International Journal of Cancer. Journal International Du Cancer
M A NagaiR R Brentani

Abstract

In this study we investigated the incidence of mutations and loss of heterozygosity (LOH) of the TP53 gene in DNA samples from paired tumor and adjacent normal tissue from 90 patients with untreated squamous-cell carcinoma of the head and neck. Evidence for TP53 mutations were demonstrated in 53% (48/90) of the cases analyzed. All cases were also examined for loss of heterozygosity, using a PCR-based polymorphic marker at TP53. LOH was found in 36 out of 72 (50%) informative cases. Direct sequencing of PCR products was performed in 45 cases with evidence of mutations. The sequencing results revealed the presence of base-substitutions (67%), deletions (29%) and insertions (4%). Of the base-substitutions, 70% were transitions and 30% were transversions. Demographic variables, tumor site, stage (TNM), family history of cancer, lymph-node involvement and histological grade were not important predictors of TP53 mutations. Nor did TP53 genetic alterations correlate with survival status. In conclusion, we show that TP53 genetic alterations are frequent in head-and-neck tumors, but are not associated with clinicopathological variables or disease progression. Our study provides an evaluation of the spectrum of TP53 mutations in the path...Continue Reading

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Citations

Nov 7, 2006·Experimental and Molecular Pathology·Valéria Cristina da Costa AndradeBernardo Garicochea
Jun 8, 2014·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Aiko NobusawaTetsunari Oyama
Aug 24, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M A Nagai
Dec 29, 2000·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·E C MiraccaM A Nagai
Mar 16, 2000·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·A F LogulloA J Simpson

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