TP53 Mutations in Serum Circulating Cell-Free Tumor DNA As Longitudinal Biomarker for High-Grade Serous Ovarian Cancer.

Biomolecules
Silvia R VitaleMaurice P H M Jansen

Abstract

The aim of this study was to determine an optimal workflow to detect TP53 mutations in baseline and longitudinal serum cell free DNA (cfDNA) from high-grade serous ovarian carcinomas (HGSOC) patients and to define whether TP53 mutations are suitable as biomarker for disease. TP53 was investigated in tissue and archived serum from 20 HGSOC patients by a next-generation sequencing (NGS) workflow alone or combined with digital PCR (dPCR). AmpliSeq™-focused NGS panels and customized dPCR assays were used for tissue DNA and longitudinal cfDNAs, and Oncomine NGS panel with molecular barcoding was used for baseline cfDNAs. TP53 missense mutations were observed in 17 tissue specimens and in baseline cfDNA for 4/8 patients by AmpliSeq, 6/9 patients by Oncomine, and 4/6 patients by dPCR. Mutations in cfDNA were detected in 4/6 patients with residual disease and 3/4 patients with disease progression within six months, compared to 5/11 patients with no residual disease and 6/13 patients with progression after six months. Finally, mutations were detected at progression in 5/6 patients, but not during chemotherapy. NGS with molecular barcoding and dPCR were most optimal workflows to detect TP53 mutations in baseline and longitudinal serum cf...Continue Reading

References

May 18, 2001·Nature·G I Evan, K H Vousden
Mar 7, 2002·Breast Cancer Research : BCR·Milena GascoTim Crook
Mar 6, 2003·Human Mutation·Monique Schuijer, Els M J J Berns
Aug 18, 2005·British Journal of Cancer·L M McShaneUNKNOWN Statistics Subcommittee of the NCI-EORTC Working Group on Cancer Diagnostics
Sep 10, 2005·American Journal of Obstetrics and Gynecology·Elizabeth M SwisherMary-Claire King
Sep 14, 2006·Cancer Biology & Therapy·Aparna A KamatAnil K Sood
Dec 2, 2006·Biochimica Et Biophysica Acta·M Fleischhacker, B Schmidt
Feb 28, 2008·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Elisa CapizziMichelangelo Fiorentino
Dec 17, 2009·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Ruifeng ZhangKejing Ying
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jennifer P MortonOwen J Sansom
Mar 17, 2010·The Journal of Pathology·Ahmed Ashour AhmedJames D Brenton
Nov 26, 2010·Annals of Oncology : Official Journal of the European Society for Medical Oncology·B DobrzyckaL Chyczewski
May 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Isaac KindeBert Vogelstein
May 18, 2012·Cancer Research·Els M J J Berns, David D Bowtell
Jan 23, 2014·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Luis A Diaz, Alberto Bardelli
Oct 24, 2015·The European Respiratory Journal·Claire TissotSébastien Couraud
Mar 26, 2016·Biochimica Et Biophysica Acta·Jenna VanLiere Canzoniero, Ben Ho Park
Jun 9, 2016·Oncotarget·Maurice Phm JansenEls Mjj Berns
Jul 17, 2016·Molecular Cancer Research : MCR·Stanislav VolikColin Collins
Aug 21, 2016·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Sarah CargninSalvatore Terrazzino
Nov 15, 2016·The Journal of Pathology. Clinical Research·Martin KöbelJames D Brenton
Feb 19, 2017·Advances in Clinical Chemistry·G PerkinsV Taly
Jun 22, 2017·International Journal of Molecular Sciences·Livia ManzellaPaolo Vigneri
Jul 29, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Lydia GiannopoulouEvi S Lianidou
Nov 9, 2017·Expert Review of Molecular Diagnostics·Mathilde PostelShu-Fang Wang-Renault
Oct 9, 2018·The Journal of Molecular Diagnostics : JMD·Silvia R VitaleJohn W M Martens
Dec 6, 2018·Molecular Oncology·Lisanne F van DesselMartijn P Lolkema
Sep 11, 2019·The Journal of Obstetrics and Gynaecology Research·Zhou YuHaidong Wang

❮ Previous
Next ❯

Citations

Sep 5, 2020·Critical Reviews in Microbiology·Vincent De MaesschalckYves Briers
Oct 11, 2020·Journal of Hematology & Oncology·Meinusha GovindarajanThomas Kislinger
Feb 7, 2021·Biomolecules·Gabriella D'Orazi
Aug 8, 2021·International Journal of Molecular Sciences·Bárbara A Mc CormackJuan Gilabert-Estellés
Jul 3, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Emmanuelle JeannotSuzy Scholl

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ampliseq
genotyping
Chip
biopsies
biopsy

Software Mentioned

Torrent Suite
ANNOVAR
Galaxy
Ampliseq
Oncomine
Integrative Genomics Viewer
Variant Caller

Related Concepts

Related Feeds

Cancer Sequencing

Several sequencing approaches are employed to understand and examine tumor development and progression. These include whole genome as well as RNA sequencing. Here is the latest research on cancer sequencing.