Tpcn2 knockout mice have improved insulin sensitivity and are protected against high-fat diet-induced weight gain.

Physiological Genomics
Hong HeLeah C Solberg Woods

Abstract

Type 2 diabetes is a complex disorder affected by multiple genes and the environment. Our laboratory has shown that in response to a glucose challenge, two-pore channel 2 ( Tpcn2) knockout mice exhibit a decreased insulin response but normal glucose clearance, suggesting they have improved insulin sensitivity compared with wild-type mice. We tested the hypothesis that improved insulin sensitivity in Tpcn2 knockout mice would protect against the negative effects of a high fat diet. Male and female Tpcn2 knockout (KO), heterozygous (Het), and wild-type (WT) mice were fed a low-fat (LF) or high-fat (HF) diet for 24 wk. HF diet significantly increases body weight in WT mice relative to those on the LF diet; this HF diet-induced increase in body weight is blunted in the Het and KO mice. Despite the protection against diet-induced weight gain, however, Tpcn2 KO mice are not protected against HF-diet-induced changes in glucose or insulin area under the curve during glucose tolerance tests in female mice, while HF diet has no significant effect on glucose tolerance in the male mice, regardless of genotype. Glucose disappearance during an insulin tolerance test is augmented in male KO mice, consistent with our previous findings suggesti...Continue Reading

References

Jun 1, 1992·The American Journal of Physiology·D B WestR L Atkinson
Sep 1, 1988·Diabetes·R S SurwitM N Feinglos
Feb 12, 2008·Current Protocols in Molecular Biology·Sami HeikkinenJohan Auwerx
Apr 10, 2008·Diabetes·Eric D BerglundDavid H Wasserman
Jul 29, 2008·Transplant International : Official Journal of the European Society for Organ Transplantation·Jordi RoviraFritz Diekmann
Apr 24, 2009·Nature·Peter J CalcraftMichael X Zhu
Jan 14, 2010·Physiological Genomics·Leah C Solberg WoodsWilliam Valdar
Aug 18, 2010·Disease Models & Mechanisms·Julio E AyalaUNKNOWN NIH Mouse Metabolic Phenotyping Center Consortium
Jan 22, 2011·Journal of Biomedicine & Biotechnology·Sunil K Panchal, Lindsay Brown
Sep 6, 2012·Physiological Genomics·Leah C Solberg WoodsWilliam Valdar
Oct 10, 2012·Nature Reviews. Genetics·Jonathan Flint, Eleazar Eskin
Sep 6, 2013·Genome Research·Michael J FlisterCarol Moreno
Aug 22, 2014·Nature Communications·Christian GrimmChristian Wahl-Schott
Sep 23, 2014·Genetics·Shirng-Wern TsaihLeah C Solberg Woods
Nov 10, 2015·Nature Genetics·Kyle J GaultonUNKNOWN DIAbetes Genetics Replication And Meta-analysis (DIAGRAM) Consortium
Dec 25, 2015·Trends in Pharmacological Sciences·Huan CaiFeng Liu
Jan 13, 2016·The Journal of Physiology·Vanessa García-RúaFrancisca Lago
May 18, 2016·European Journal of Human Genetics : EJHG·James P Cook, Andrew P Morris
Jun 23, 2016·Messenger·Margarida RuasJohn Parrington
Nov 16, 2016·Science·Paul W Franks, Mark I McCarthy
Jun 2, 2017·Diabetes·Robert A ScottUNKNOWN DIAbetes Genetics Replication And Meta-analysis (DIAGRAM) Consortium

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Methods Mentioned

BETA
gene knockout
gene trap
PCR
ELISA

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