TPP riboswitch-dependent regulation of an ancient thiamin transporter in Candida

PLoS Genetics
Paul D DonovanGeraldine Butler

Abstract

Riboswitches are non-coding RNA molecules that regulate gene expression by binding to specific ligands. They are primarily found in bacteria. However, one riboswitch type, the thiamin pyrophosphate (TPP) riboswitch, has also been described in some plants, marine protists and fungi. We find that riboswitches are widespread in the budding yeasts (Saccharomycotina), and they are most common in homologs of DUR31, originally described as a spermidine transporter. We show that DUR31 (an ortholog of N. crassa gene NCU01977) encodes a thiamin transporter in Candida species. Using an RFP/riboswitch expression system, we show that the functional elements of the riboswitch are contained within the native intron of DUR31 from Candida parapsilosis, and that the riboswitch regulates splicing in a thiamin-dependent manner when RFP is constitutively expressed. The DUR31 gene has been lost from Saccharomyces, and may have been displaced by an alternative thiamin transporter. TPP riboswitches are also present in other putative transporters in yeasts and filamentous fungi. However, they are rare in thiamin biosynthesis genes THI4 and THI5 in the Saccharomycotina, and have been lost from all genes in the sequenced species in the family Saccharomyc...Continue Reading

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Citations

Nov 21, 2018·Current Genetics·Bronislava BrejováJozef Nosek
Oct 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Carla Gonçalves, Paula Gonçalves
Jul 28, 2020·International Journal of Molecular Sciences·Katarzyna Taylor, Krzysztof Sobczak
Jan 21, 2021·Antibiotics·Vipul Panchal, Ruth Brenk
Apr 4, 2021·Life·Huanhuan Ge, Mario Andrea Marchisio

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Methods Mentioned

BETA
Fluorescence
PCR
RNA-seq

Software Mentioned

Coffee
Candida Gene Order Browser
HMMER
Pico
Infernal
Gblocks
SeaView
RAxML
cmsearch
Muscle

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