TPX2 in malignantly transformed human bronchial epithelial cells by anti-benzo[a]pyrene-7,8-diol-9,10-epoxide

Toxicology
Lijuan ZhangYuedan Wang

Abstract

In order to elucidate the function of the targeting protein for Xenopus kinesin-like protein 2 (Xklp2) (TPX2) in the malignant transformation of human bronchial epithelial cells induced by anti-benzo[a]pyrene-trans-7, 8-dihydrodiol-9, 10-epoxide (anti-BPDE), TPX2 was characterized in cells at both the gene and the protein levels. TPX2 was present at higher levels in 16HBE-C cells than in 16HBE cells as demonstrated by two-dimensional gel electrophoresis, immunocytochemistry, Western blot analysis and RT-PCR. TPX2 was also detected in lung squamous-cell carcinoma tissues by immunohistochemistry, but not in normal lung tissues. Depression of TPX2 by RNA interference in 16HBE-C cells led to a decrease in cell proliferation, S-phase cell cycle arrest and cell apoptosis. Abnormal TPX2 tyrosine phosphorylation was detected in 16HBE-C cells, and this could be inhibited, to different degrees, by tyrosine kinase inhibitors. Inhibiting tyrosine phosphorylation in 16HBE-C cells by three selected tyrosine protein kinase inhibitors, tyrphostin 47, AG112 and AG555, caused G(0)/G(1)-phase cell cycle arrest. Our results suggest that anti-BPDE can cause the over-expression of TPX2 and its aberrant tyrosine phosphorylation. Misregulation of TPX2...Continue Reading

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Citations

Feb 22, 2014·Cellular and Molecular Life Sciences : CMLS·Gernot NeumayerMinh Dang Nguyen
Sep 23, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hong-Chun LiuJun-Wen Liu
Jan 10, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Peng ZhaoZongcan Zhou
Nov 18, 2014·PloS One·Rossana CocchiolaMargherita Eufemi
Apr 11, 2014·Molecular Medicine Reports·Peiyue JiangTongjun Liu
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yan YaoYuegang Wei

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