Trace determination of anthracyclines in urine: a new high-performance liquid chromatography/tandem mass spectrometry method for assessing exposure of hospital personnel

Rapid Communications in Mass Spectrometry : RCM
C SottaniC Minoia

Abstract

Health-care workers handling antineoplastic agents may be exposed to extremely low doses of these drugs. Very sensitive and specific analytical methods are therefore needed for biological monitoring. The aim of this study was to develop and validate a method for trace level determination of doxorubicin, epirubicin, daunorubicin and idarubicin in human urine, using epi-daunorubicin as an internal standard. Solid-phase extraction (SPE) was used for sample preparation. Urine samples were loaded onto Bond Elut C18 cartridges. The analytes were eluted in methylene chloride/2-propanol (1:1, v/v) and then evaporated to dryness. The residue was reconstituted with the mobile phase prior to high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) analysis. Quantitation of each analyte was performed using the multiple reaction monitoring (MRM) method. The urine assay was linear over the range 0.1-2.0 microg/L, with a lower limit of quantification (LLOQ) of 0.10 microg/L for doxorubicin and epirubicin, and 0.03 microg/L for daunorubicin and idarubicin. The respective limits of detection (LODs) were 0.04 and 0.01 microg/L. The precision and accuracy of the assay were determined on three different days. The within-series ...Continue Reading

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Citations

Jun 10, 2014·Journal of Photochemistry and Photobiology. B, Biology·Can Ozluer, Hayriye Eda Satana Kara
May 19, 2012·Analytica Chimica Acta·Fernando Martínez FerrerasHans H Gorris
Aug 16, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kristof E MaudensWilly E Lambert
Jun 7, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Petra KrumpochovaPetr Mlejnek
May 19, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Cristina SottaniClaudio Minoia
Sep 29, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Cristina SottaniClaudio Minoia
Oct 4, 2011·Talanta·Susanne NussbaumerSandrine Fleury-Souverain
Dec 17, 2016·Journal of the American Society for Mass Spectrometry·Areum HongHugh I Kim
Dec 25, 2010·Journal of Oncology Pharmacy Practice : Official Publication of the International Society of Oncology Pharmacy Practitioners·Jack R PrettyD Gayle Debord
Jun 1, 2017·The Analyst·Nicolas GuichardSandrine Fleury-Souverain
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Sep 5, 2019·Journal of Oncology Pharmacy Practice : Official Publication of the International Society of Oncology Pharmacy Practitioners·Pan ShuManjunath P Pai
Dec 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Olga MaliszewskaAlina Plenis
Sep 29, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Antoine VillaMireille Canal-Raffin

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