Trace determination of antibacterial pharmaceuticals in fishes by microwave-assisted extraction and solid-phase purification combined with dispersive liquid-liquid microextraction followed by ultra-high performance liquid chromatography-tandem mass spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Peiting HuangNing Liang

Abstract

A novel pretreatment method involving microwave-assisted extraction and solid-phase purification combined with dispersive liquid-liquid microextraction (MAE-SPP-DLLME) followed by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was established for the simultaneous determination of six antibacterial pharmaceuticals including metronidazole, tinidazole, chloramphenicol, thiamphenicol, malachite green and crystal violet. The conditions of MAE were optimized using an orthogonal design and the optimal conditions were found to be 8mL for acetonitrile, 50°C for 5min. Then, neutral alumina column was employed in the solid-phase purification. Finally, the critical parameters affecting DLLME, including selection of extraction and dispersive solvent, adjustment of pH, salt concentration, extraction time, were investigated by single factor study. Under optimum conditions, good linearities (r>0.9991) and satisfied recoveries (Recoveries>87.0%, relative standard deviation (RSD)<6.3%) were observed for all of the target analytes. The limits of detection and quantification were 4.54-101.3pgkg(-1) and 18.02-349.1pgkg(-1), respectively. Intra-day and inter-day RSDs were all lower than 3.6%. An obvious reduction...Continue Reading

References

Feb 24, 2001·Journal of Chromatography. a·C S Eskilsson, E Björklund
Apr 11, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M F FestingJ A Turton
May 8, 2004·Aquatic Toxicology·Shivaji SrivastavaD Roy
Mar 24, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sandra J CulpFrederick A Beland
Apr 1, 2006·Journal of Chromatography. a·Mohammad RezaeeSana Berijani
Jun 20, 2006·Mass Spectrometry Reviews·W M A NiessenR Andreoli
Dec 7, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Baomi LiuYuru Geng
Aug 9, 2011·Journal of Chromatography. a·Chung-Hung ChanFabian Wai-Lee Kung
Nov 22, 2013·Analytical and Bioanalytical Chemistry·Mohammad Saraji, Malihe Khalili Boroujeni

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Citations

Mar 16, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yi ZhangJiong Wang
Sep 21, 2016·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Glenn LoosDeirdre Cabooter

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