PMID: 9657857Jul 10, 1998Paper

Traced orthologous amplified sequence tags (TOASTs) and mammalian comparative maps

Mammalian Genome : Official Journal of the International Mammalian Genome Society
Z JiangF Galibert

Abstract

Progress on mammalian comparative maps could be significantly accelerated by developing reagents defining orthologous landmarks in the genome of many mammalian species. Using the large databases of gene sequences, we designed 225 orthologous gene-specific primer pairs corresponding to 146 functional genes. Of these 225 primer pairs, 155 (68.9%), 182 (80.9%), 126 (56.0%), and 82 (36.4%) produced a single PCR product when tested against human, pig, dog, and hamster genomic DNA, respectively. In addition to the general rules of primer designing, particular factors must be taken into consideration when choosing gene-specific universal primers--for instance, preference for single-exon regions or highly conserved segments among species, avoidance of GC-rich regions. Sequencing all the canine PCR products traced by these primers demonstrated that of 123 traced canine fragments with readable and reliable sequences, 121 (98.4%) were found to match the GenBank orthologous sequences used for designing the primers, after a BLAST search. Comparative characterization of PCR fragments among human, pig, dog, and hamster revealed that the length of a single exon was much conserved among species, with few exceptions. As the fragments were traced...Continue Reading

Citations

Jan 18, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Clifton M RamsdellMichael J Dewey
Feb 26, 2000·Trends in Genetics : TIG·E A OstranderD F Patterson
Jul 25, 2003·Molecular Phylogenetics and Evolution·Heather Amrine-MadsenMark S Springer
Mar 18, 2000·Animal Biotechnology·M YerleJ Gellin
Jan 10, 2003·Nucleic Acids Research·Emmanuelle KarsentyJoël Gellin
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Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Terje RaudseppBhanu P Chowdhary
Sep 14, 2004·Médecine sciences : M/S·Francis GalibertChristophe Hitte
Aug 25, 2009·Molecular Phylogenetics and Evolution·Jun J SatoHitoshi Suzuki
May 15, 2010·Molecular Ecology·Robert C ThomsonJarrett R Johnson
Dec 19, 2006·Molecular Phylogenetics and Evolution·Klaus-Peter KoepfliRobert K Wayne
Mar 1, 2006·Molecular Phylogenetics and Evolution·Klaus-Peter KoepfliRobert K Wayne
Oct 3, 2002·Annual Review of Genetics·Stephen J O'BrienNaoya Yuhki
Jan 13, 2005·Genome Génome / Conseil National De Recherches Canada·Zhihua JiangRaymond W Wright
Aug 15, 2002·Genome Génome / Conseil National De Recherches Canada·Zhihua JiangAnn M Verrinder Gibbins
Jan 14, 2021·Heliyon·Giovanni ForcinaJennifer A Leonard

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