Tracer diffusion in F-actin and Ficoll mixtures. Toward a model for cytoplasm

Biophysical Journal
L HouK Luby-Phelps

Abstract

We have previously reported that self-diffusion of inert tracer particles in the cytoplasm of living Swiss 3T3 cells is hindered in a size-dependent manner (Luby-Phelps, K., D.L. Taylor, and F. Lanni. 1986. J. Cell Biol. 102:2015-2022; Luby-Phelps, K., P.E. Castle, D.L. Taylor, and F. Lanni. 1987. Proc Natl. Acad. Sci. USA. 84:4910-4913). Lacking a theory that completely explains our data, we are attempting to understand the molecular architecture responsible for this phenomenon by studying tracer diffusion in simple, reconstituted model systems. This report contains our findings on tracer diffusion in concentrated solutions of Ficoll 70 or Ficoll 400, in solutions of entangled F-actin filaments, and in solutions of entangled F-actin containing a background of concentrated Ficoll particles or concentrated bovine serum albumin (BSA). A series of size-fractionated fluorescein-Ficolls were used as tracer particles. By fluorescence recovery after photobleaching (FRAP), we obtained the mean diffusion coefficients in a dilute, aqueous reference phase (Do), the mean diffusion coefficients in the model matrices (D), and the mean hydrodynamic radii (RH) for selected tracer fractions. For each model matrix, the results were compared with...Continue Reading

References

Jul 1, 1979·The Journal of Cell Biology·J J Wolosewick, K R Porter
Nov 5, 1979·Journal of Molecular Biology·J H Hartwig, T P Stossel
Jun 1, 1986·The Journal of Cell Biology·K Luby-PhelpsF Lanni
Jan 1, 1988·Cell Motility and the Cytoskeleton·J R SimonD L Taylor
Jan 1, 1985·Annual Review of Cell Biology·T P StosselH L Yin
Jan 1, 1988·Cell Motility and the Cytoskeleton·K Luby-Phelps, D L Taylor
Jan 1, 1988·Annual Review of Biophysics and Biophysical Chemistry·K Luby-PhelpsD L Taylor
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K Luby-PhelpsF Lanni
Jan 1, 1986·Annual Review of Biochemistry·T D Pollard, J A Cooper
May 29, 1987·Biochemical and Biophysical Research Communications·J F Casella, D J Maack
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N D GershonB L Trus
Nov 1, 1974·Analytical Biochemistry·T W Houk, K Ue
Apr 1, 1970·Journal of Theoretical Biology·F Oosawa
Mar 22, 1967·Biochimica Et Biophysica Acta·T C Laurent, K A Granath
Mar 22, 1967·Biochimica Et Biophysica Acta·T C Laurent
Jul 1, 1984·The Journal of Cell Biology·K R Porter
Nov 4, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D L Taylor, M Fechheimer
Sep 1, 1982·Cell·A B Fulton
Jul 1, 1981·The Journal of Cell Biology·M Schliwa, J van Blerkom
Oct 1, 1982·Biophysical Journal·J YguerabideE E Yguerabide
Jan 1, 1961·Biophysical Journal·M A LAUFFER

❮ Previous
Next ❯

Citations

Apr 14, 2006·European Biophysics Journal : EBJ·Daniel LavaletteAntonie J Visser
Jan 1, 1996·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·P Todd, D M Klaus
Dec 30, 2009·The Journal of Physical Chemistry. B·Hugo Sanabria, M Neal Waxham
Jan 1, 1993·The Journal of Cell Biology·H P KaoA S Verkman
Feb 24, 2010·The Journal of General Physiology·Peter D CalvertEdward N Pugh
Jun 28, 2012·EURASIP Journal on Bioinformatics & Systems Biology·Noriko Hiroi1Heinz Koeppl
Oct 24, 2009·PloS One·Ronald Hancock, Yasmina Hadj-Sahraoui
Jun 30, 1997·The Journal of Cell Biology·J R AbneyB A Scalettar
Jul 14, 1997·The Journal of Cell Biology·O SeksekA S Verkman
Jan 20, 2009·Journal of Theoretical Biology·Cassie S Mitchell, Robert H Lee
Sep 30, 2006·Biophysical Chemistry·Silvia ZorrillaM Pilar Lillo
Jun 2, 2009·Advances in Colloid and Interface Science·Niklas LorénAnne-Marie Hermansson
Feb 17, 2009·Biophysical Journal·Jun Soo Kim, Arun Yethiraj
Jun 17, 2009·Biophysical Journal·Michael T KlannMatthias Reuss
Dec 10, 1999·Biophysical Journal·J R Wenner, V A Bloomfield
Feb 1, 1994·Current Opinion in Cell Biology·K Luby-Phelps
Oct 22, 2017·Bioscience Reports·Haiqing BaiDavid A Dean
Dec 12, 2001·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·M T ValentineD A Weitz
Dec 1, 1996·Cell Biochemistry and Function·H Knull, A P Minton
Oct 15, 2008·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Byoungkoo LeeRussell Schwartz
Sep 15, 1995·The Journal of Biological Chemistry·K Luby-PhelpsD Won
Jan 1, 1995·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·L S LuoH Gould
Apr 1, 1994·The American Journal of Physiology·M M Garner, M B Burg
Jul 7, 2021·Life Science Alliance·Beatrice Terni, Artur Llobet
Feb 13, 2001·Biophysical Journal·D A Smith, R M Simmons

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