Tracheoplasty with cartilage-engineered esophagus environments

Journal of Pediatric Surgery
Makoto KomuraTadashi Iwanaka

Abstract

Our objective was to investigate the feasibility of engineering cartilage on the esophagus layer and outside the esophagus. Moreover, we investigated the feasibility of tracheoplasty with cartilage engineered on the esophagus in rabbits. Chondrocytes were isolated from auricular cartilages. 1. Engineered cartilage formation by histological findings on/into the esophageal layer was compared with that of injectable scaffold and preformed scaffold with chondrocytes. 2. Chondrocytes adhered to gelatin+vicryl mesh™ and b-FGF, were implanted on the outer esophageal surface. Four weeks after seeding, we found that cartilage was implanted in the midposterior portion of the cervical trachea (n=5), and it was retrieved 8weeks after seeding. 1. A gelatin sponge incorporating β-TCP with vicryl mesh™ showed the best performance for fabricating engineered cartilage on the outer side of the esophagus. 2. Two of 5 rabbits died due to obstructed esophagus. Cartilage engineered outside the esophagus by a composite scaffold as the main material in the gelatin sponge, maintained the airway structure for up to 1month after implantation. Tracheal epithelial regeneration occurred in the internal lumen of this engineered cartilage. Tracheoplasty with ...Continue Reading

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Feb 18, 2014·Journal of Pediatric Surgery·Makoto KomuraTadashi Iwanaka

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Citations

Jul 15, 2016·The Annals of Otology, Rhinology, and Laryngology·Tendy ChiangChristopher K Breuer
Nov 28, 2017·Current Stem Cell Reports·Elizabeth F MaughanPaolo De Coppi
Nov 3, 2016·Current Opinion in Otolaryngology & Head and Neck Surgery·Sharon H Gnagi, David R White
Aug 31, 2020·Laryngoscope Investigative Otolaryngology·Weston L NiermeyerTendy Chiang

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