Tracing lymphopoiesis with the aid of a pTalpha-controlled reporter gene

Nature Immunology
Fotini GounariBoris Reizis

Abstract

A transgenic reporter mouse strain, which expressed the human CD25 (hCD25) surface marker as a reporter under the control of the pre-T cell receptor alpha(pTalpha) promoter, was used to identify lymphoid precursors that expressed pTalpha intracellularly. The hCD25 reporter marked intra- and extrathymic precursors of lymphocytes but not myeloid cells. The earliest intrathymic precursors were CD4(lo)CD8(-)CD25(-)CD44(+)c-Kit(+) cells that expressed elevated levels of Notch-1 mRNA. Clonogenic assays showed that the extrathymic precursors were common lymphoid progenitors (CLPs) that included CD19(-), B220(+), Thy1(+) and CD4(+) cells. Thus, the pTalpha reporter can be used to trace lymphopoiesis between CLPs and alphabeta T cells. The slower extinction of the hCD25 reporter compared to pTalpha enabled us to define points at which pTalpha(-) lineages branched off.

References

Dec 1, 1991·The Journal of Experimental Medicine·L WuK Shortman
Jul 1, 1988·Science·G J SpangrudeI L Weissman
Jul 1, 1995·European Journal of Immunology·L BrunoH R Rodewald
Oct 1, 1993·The Journal of Experimental Medicine·Y MatsuzakiH Nakauchi
Nov 18, 1994·Science·C Saint-RufH von Boehmer
Jan 1, 1996·Annual Review of Immunology·K Shortman, L Wu
Feb 17, 1997·The Journal of Experimental Medicine·I L MårtenssonT H Winkler
Mar 3, 1997·The Journal of Experimental Medicine·L BrunoH Spits
Feb 25, 1998·Stem Cells·R C Fisher, E W Scott
Mar 13, 2001·Annual Review of Immunology·R R Hardy, K Hayakawa
Jul 4, 2001·The Journal of Experimental Medicine·D AllmanW S Pear
Oct 3, 2001·The Journal of Experimental Medicine·A C JalecoL Parreira
Nov 1, 2001·Annual Review of Cell and Developmental Biology·I L WeissmanF Gage
Nov 20, 2001·Nature Immunology·C Asselin-PaturelG Trinchieri
Mar 27, 2002·Nature Reviews. Immunology·Katia Georgopoulos

❮ Previous
Next ❯

Citations

Mar 16, 2005·Current Opinion in Immunology·Rosana PelayoPaul W Kincade
Dec 14, 2002·Human Immunology·Francine BrièreElizabeth E M Bates
Mar 14, 2003·Current Opinion in Immunology·Luigi A Warren, Ellen V Rothenberg
Apr 12, 2003·Seminars in Immunology·Warren S Pear, Freddy Radtke
Dec 10, 2013·Nature Immunology·Cyrille RamondAna Cumano
Aug 10, 2004·Nature Immunology·Benjamin A Schwarz, Avinash Bhandoola
Jul 19, 2005·Nature Immunology·Fotini GounariKhashayarsha Khazaie
Jul 7, 2005·Nature Reviews. Immunology·Harald von Boehmer
Dec 22, 2007·Nature Reviews. Immunology·Ellen V RothenbergMary A Yui
Oct 16, 2007·Oncogene·H Iwasaki, K Akashi
Nov 24, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kristine GermarFotini Gounari
Mar 16, 2005·The Journal of Experimental Medicine·Sheila DiasPaulo Vieira
Jun 29, 2005·The Journal of Experimental Medicine·Claudia Benz, Conrad C Bleul
Jul 19, 2006·The Journal of Experimental Medicine·Andreas KruegerHarald von Boehmer
Jan 11, 2007·Molecular and Cellular Biology·Holger BabbeBoris Reizis
Apr 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·M Lucila ScimoneUlrich H von Andrian
Jul 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jian XuStephen T Smale
May 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Florian KleinMarkus Müschen
Sep 15, 2005·International Journal of Hematology·Toshihiro Miyamoto, Koichi Akashi
Nov 28, 2006·Trends in Immunology·Sho Yamasaki, Takashi Saito
Apr 22, 2011·Blood·Miaofen G HuPhilip W Hinds
May 31, 2007·Current Opinion in Hematology·Ellen V Rothenberg
Nov 5, 2002·Nature Reviews. Immunology·Rod Ceredig, Ton Rolink
Feb 24, 2006·Nature Reviews. Immunology·Avinash Bhandoola, Arivazhagan Sambandam
Feb 2, 2006·Immunological Reviews·Benjamin A Schwarz, Avinash Bhandoola
Feb 2, 2006·Immunological Reviews·Elizabeth-Sharon David-FungEllen V Rothenberg
Feb 2, 2006·Immunological Reviews·Ioana VisanCynthia J Guidos
Feb 2, 2006·Immunological Reviews·Michele K Anderson
Feb 2, 2006·Immunological Reviews·Sandra M Hayes, Paul E Love
Mar 31, 2007·Annals of the New York Academy of Sciences·Jae-Yong KwakGerald J Spangrude
Feb 5, 2003·The Journal of Biological Chemistry·Mathieu TremblayTrang Hoang
Oct 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shawn P FahlTimothy P Bender
Feb 11, 2015·Annual Review of Immunology·Maria Elena De Obaldia, Avinash Bhandoola
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·S Scott PerryXiao-Hong Sun
Jun 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·C Chace TydellEllen V Rothenberg
Feb 25, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Veena KrishnamoorthyBarbara L Kee
Sep 13, 2003·Immunological Reviews·Jason GillRichard Boyd
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·David GerberPablo Pereira
Aug 14, 2012·Genesis : the Journal of Genetics and Development·Katayoun AghajaniFotini Gounari

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.